3D-NHS Microplates
for covalent coupling of biomolecules via their primary amine groups
The NHS-esters react immediately with primary amine (-NH2) groups of biochemical species to bind them covalently to the microplate surface. However, due to its high reactivity the NHS ester is susceptible to hydrolysis, and thus, should be processed quickly.
Key Features
- Highly reactive surface for fast coupling of primary amines
- A direct, simple approach to covalently attach molecules to the well surface of a microplate
PolyAn also equips glass slides, coverslips, polymer slides, and COP films with 3D-NHS surfaces. Please do not hesitate to contact us, if you would like to functionalize a different format or substrate with our 3D-NHS surface.
Selected Publications
- Vellecco, V. et al., `Phosphodiesterases S-sulfhydration contributes to human skeletal muscle function´, Pharmacol Research, 2022, 177, 106108. DOI: 10.1016/j.phrs.2022.106108.
- Santur, K. et al., `Ligand-Induced Stabilization of the Native Human Superoxide Dismutase 1´, ACS Chem Neurosci, 2021, 12, 2520. DOI: 10.1021/acschemneuro.1c00253.
- Zivanovic, J. et al., `Selective Persulfide Detection Reveals Evolutionarily Conserved Antiaging Effects of S-Sulfhydration´, Cell Metabolism, 2019, 30, 1152. DOI: 10.1016/j.cmet.2019.10.007.
- Veugelen, S. et al., `Screening and Characterization Strategies for Nanobodies Targeting Membrane Proteins´, Methods in Enzymology, 2017, 584, 59. DOI: 10.1016/bs.mie.2016.10.029.