Fluorescent Microparticles
PolyAn offers a wide range of fluorescence encoded PMMA beads and functionalized PMMA beads. Applications include multiplex bead assays, calibration of flow cytometers, calibration of fluorescence microscopes, and fluorescence lifetime-based applications. All microparticles are available in sizes between 2–20 µm. PolyAn also offers monodisperse, fluorescence encoded PMMA nanoparticles in the 100 – 500 nm size range. Please note, that it is possible to tailor the fluorescence intensity to your specific requirements. PolyAn also produces customized microparticles which incorporate fluorophores for other spectral ranges.
PolyAn's fluorescent beads can be equipped with a wide range of functional surfaces:
- Unmodified fluorescent beads or modified with a Low aggregation surface
- Reactive surfaces: 3D-Carboxy and 3D-Aldehyde
- Surfaces for Click chemistry: 3D-Azide, 3D-Alkyne, 3D-DBCO, and 3D-MTZ
- Functionalized with Streptavidin, Neutravidin, or Protein A/G
A custom modification with other functional groups (e.g. Maleimide, Biotin,…), with different loading capacities, and the customised coupling of biomolecules (oligonucleotides, peptides, antibodies …) are also available upon request. Please do not hesitate to contact us!
Fluorescence encoding toolbox

With PolyAn’s production process the fluorophores are incorporated into the beads during the polymerization process. This ensures a much more homogeneous distribution of the dyes within the beads when compared to conventional diffusion controlled dyeing processes. Additionally, the fluorophores are caged within the polymeric matrix and are less likely to leak-out. Please have a look at our fluorescence encoded products.
Selected Publications and References
- Pham, N.M. et al., `Immuno-gold silver staining assays on capillary-driven microfluidics for the detection of malaria antigens´, Biomed. Microdevices, 2019, 21, 24. DOI: 10.1007/s10544-019-0376.
- Pham, N.M. et al., `A bead-based immunogold-silver staining assay on capillary-driven microfluidics´, Biomed. Microdevices, 2018, 20, 41. DOI: 10.1007/s10544-018-0284-6.
- Schauer, O. et al., `Motility and chemotaxis of bacteria-driven microswimmers fabricated using antigen 43-mediated biotin display´, Sci. Rep., 2018, 8, 9801. DOI: 10.1038/s41598-018-28102-9.
- Mostaghaci, B. et al., `Bioadhesive Bacterial Microswimmers for Targeted Drug Delivery in the Urinary and Gastrointestinal Tracts´, Adv. Sci., 2017, 4, 1700058. DOI: 10.1002/advs.201700058.
