for non-covalent coupling of positively charged biochemical species via electrostatical adsorption
An adsorptive immobilization is a non-covalent coupling method on solid supports which is realized by electrostatic, Van der Waals interactions, hydrogen bonds and hydrophobic interactions of the reactants, respectively. An electrostatic interaction is formed by an ion-ion-interaction between the surface and the applied biochemical species. The dissociation energy for typical electrostatic bond is 130 kJ/mol. It is about a third of the strength of an average covalent bond. In order to achieve an optimal adhesion the probe buffer und the adhesion conditions (pH-value) have to be optimized.
Please note, that the carboxy functionalities can be used for surface activation via 1-Ethyl-3-(3-dimethylaminopropyl) carbo-diimide (EDC), N-hydroxy succinimide (NHS).
PolyAn equips glass slides, coverslips and polymer slides as well as 96-well plates with 3D-Carboxy matrices. Please do not hesitate to contact us, if you would like to functionalize a different format or substrate with our 3D-Carboxy surface.