Fluorescent PMMA Microparticles

PolyAn's fluorescent microparticles are available in various sizes, emission spectra and fluorescence intensities. The fluorescent PMMA microparticles are suitable for use in flow cytometry, fluorescence microscopy, phagocytosis studies and cell labelling. They can be used in image based systems as well as in other screening applications. Typical applications include calibration of flow cytometers, calibration of fluorescence microscopes and multiplex bead assays.

With PolyAn’s production process up to six fluorophores can be incorporated into the beads during the bead polymerisation process. This ensures a much more homogeneous distribution of the dyes within the beads when compared to conventional diffusion controlled dyeing processes. The fluorophores are also caged within the polymeric PMMA matrix and are less likely to leak-out.

Id
Mean Diameter
Color Labeling
Excitation Emission
Solids Content
Id Mean Diameter Color Labeling Excitation Emission Solids Content
105 40 002 2 µm PolyAn Red 510–580 nm / 570–630 nm 1.0 %
105 40 005 5 µm PolyAn Red 510–580 nm / 570–630 nm 1.0 %
105 40 009 9 µm PolyAn Red 510–580 nm / 570–630 nm 1.0 %
105 40 012 12 µm PolyAn Red 510–580 nm / 570–630 nm 1.0 %
105 40 016 16 µm PolyAn Red 510–580 nm / 570–630 nm 1.0 %
105 40 020 20 µm PolyAn Red 510–580 nm / 570–630 nm 1.0 %
105 60 002 2 µm PolyAn Green 415–480 nm / 470–550 nm 1.0 %
105 60 005 5 µm PolyAn Green 415–480 nm / 470–550 nm 1.0 %
105 60 009 9 µm PolyAn Green 415–480 nm / 470–550 nm 1.0 %
105 60 012 12 µm PolyAn Green 415–480 nm / 470–550 nm 1.0 %
105 60 016 16 µm PolyAn Green 415–480 nm / 470–550 nm 1.0 %
105 60 020 20 µm PolyAn Green 415–480 nm / 470–550 nm 1.0 %
105 70 002 2 µm PolyAn Pink 450-565 nm / 540-620 nm 1.0 %
105 70 005 5 µm PolyAn Pink 450-565 nm / 540-620 nm 1.0 %
105 70 009 9 µm PolyAn Pink 450-565 nm / 540-620 nm 1.0 %
105 70 012 12 µm PolyAn Pink 450-565 nm / 540-620 nm 1.0 %
105 70 016 16 µm PolyAn Pink 450-565 nm / 540-620 nm 1.0 %
105 70 020 20 µm PolyAn Pink 450-565 nm / 540-620 nm 1.0 %
105 89 005 5 µm PolyAn Blue 350–400 nm / 400–480 nm 1.0 %
105 89 009 9 µm PolyAn Blue 350–400 nm / 400–480 nm 1.0 %
105 89 012 12 µm PolyAn Blue 350–400 nm / 400–480 nm 1.0 %
105 89 016 16 µm PolyAn Blue 350–400 nm / 400–480 nm 1.0 %
105 89 020 20 µm PolyAn Blue 350–400 nm / 400–480 nm 1.0 %
106 00 002 2 µm PolyAn Red4 590–680 nm / 660–780 nm 1.0 %
106 00 005 5 µm PolyAn Red4 590–680 nm / 660–780 nm 1.0 %
106 00 009 9 µm PolyAn Red4 590–680 nm / 660–780 nm 1.0 %
106 00 012 12 µm PolyAn Red4 590–680 nm / 660–780 nm 1.0 %
106 00 016 16 µm PolyAn Red4 590–680 nm / 660–780 nm 1.0 %
106 00 020 20 µm PolyAn Red4 590–680 nm / 660–780 nm 1.0 %
106 10 002 2 µm PolyAn Red5 490–680 nm / 660–730 nm 1.0 %
106 10 005 5 µm PolyAn Red5 490–680 nm / 660–730 nm 1.0 %
106 10 009 9 µm PolyAn Red5 490–680 nm / 660–730 nm 1.0 %
106 10 012 12 µm PolyAn Red5 490–680 nm / 660–730 nm 1.0 %
106 10 016 16 µm PolyAn Red5 490–680 nm / 660–730 nm 1.0 %
106 10 020 20 µm PolyAn Red5 490–680 nm / 660–730 nm 1.0 %
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The standard packaging volume for our fluorescent, unmodified bead is 1.5 mL. The beads are available in any size between 2 - 20 µm. Both the fluorescence intensity and the spectral characteristics can be tailored to your specific requirements as part of our Molecular Surface Engineering Service.

Our fluorescence encoded beads can also be functionalized with 3D-Carboxy, Streptavidin, Neutravidin, Protein A/G, 3D-Azide and 3D-Alkyne surfaces. A custom modification with antibodies or oligonucleotides is available upon request. Please do not hesitate to contact us!

Selected Publications and References

  • Weber, T.A. et al., `Single-Color Barcoding for Multiplexed Hydrogel Bead-Based Immunoassays´, Appl. Mater. Interfaces, 2022, 14, 25147. DOI: 10.1021/acsami.2c04361.
  • Bergmann, S. et al., `NO Synthesis in Immune-Challenged Locust Hemocytes and Potential Signaling to the CNS´, Insects, 2021, 12, 951. DOI: 10.3390/insects12100951.
  • Rodríguez-Pena, A. et al., `Spheroscope: A custom-made miniaturized microscope for tracking tumour spheroids in microfluidic devices´, Sci. Rep., 2020, 10, 2779. DOI: 10.1038/s41598-020-59673.
  • Ahlfeld, T. et al., `Bioprinting of mineralized constructs utilizing multichannel plotting of a self-setting calcium phosphate cement and a cell-laden bioink´, Biofabrication, 2018, 10, 45002. DOI: 10.1088/1758-5090/aad36d.