3D-Carboxy functionalized microparticles
PolyAn’s 3D Carboxy microparticles with antifouling behavior are suitable for coupling of proteins, antibodies, and other amine-terminated molecules.
The activation of Carboxyl groups with EDC (1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide) and NHS (N-hydroxysuccinimide) leads to highly reactive esters, which can be easily reacted with nucleophiles, e.g. Amines or Hydrazines. The NHS-ester reacts with the NH²-groups of biochemical species to form
a covalent bond with the surface.
Key features
- Easy activation using carbodiimide EDC
- Generating NHS surface as an intermediate step, sequential coupling is possible
- Amine-containing biomolecules covalently bind to activated Carboxy surface
- Low concentration of Amine-containing biomolecules is possible
- A stable covalent bond between the surface and the biomolecules is formed, i.e. no leaching can occur
PolyAn can functionalize all the beads from our portfolio with the Carboxy surfaces:
- Transparent beads
- Fluorescence encoded microparticles
- Multiplex bead populations with 8-25 peaks for flow cytometry and fluorescence imaging systems
- Fluorescence encoded submicon beads (nanoparticles)
Please do not hesitate to contact us (mail@poly-an.de), if you have any questions regarding our products. We are looking forward to your inquiry.