PolyAn’s fluorescent PMMA Nanobeads can be applied as reporters in a lateral flow assay (stripe tests) as an alternative to the conventional colorimetric read out using for example gold nanoparticles (colloidal gold).
IgM autoantibodies with affinity for the Fc portion of IgG are well-accepted biomarkers for rheumatoid arthritis. Fluorescent PMMA Nanobeads have been used in autoimmune diagnostics to detect IgM autoantibodies in sera from patients with rheumatoid arthritis.
Principle of the Nanobead-based lateral flow assay. Test line: The fluorescence-encoded Nanobeads only accumulate at the test line, when IgM autoantibodies are present in a patient serum and bind to the immobilized human Fc fragment. Control line: The Nanobeads always accumulate at the control line, because of the binding of IgG antibodies to Protein G.
Nanobead-based lateral flow assay strips with different dilutions of serum from a patient with rheumatoid arthritis using a fluorescence scanner (excitation at 640 nm, detection at 700–750 nm).
- Schmidt, C. et al., `Fluorescence-encoded poly(methyl metharcylate) nanoparticles for a lateral flow assay detecting IgM autoantibodies in rheumatoid arthritis´, Anal. Biochem., 2021, 633, 114389. DOI: 10.1016/j.ab.2021.114389.