Microarray Slides for DNA applications

All of PolyAn’s reactive surfaces are completely transparent. They are characterized by a low lot-to-lot variation that is specified and monitored by using contact angle measurements as well as qualitative test methods.

Surface test package (5) with 3x5 polymer slides for directed immobilization of oligonucleotides

Id 104 00 926
Title Surface test package (5) with 3x5 polymer slides for directed immobilization of oligonucleotides
Substrate POLYMER
Format Planar slide
Mean Diameter
Color Labeling
Surface Modifications Streptavidin, Neutravidin and 3D-Maleimid
Solids Content
Product Dimensions 25 mm x 75 mm
Packaging
Packaging Volume 15 Slides/Pack
Package Weight
Dimensions
Hts Code 39 26 90 97
Pads Wells
Pad Size
Well Format
Product Thickness 1 mm
Description Sample package for directed coupling of oligonucleotides via Biotin-Streptavidin, Biotin-Neutravidin or Maleimid-Thiol interactions. The package is comprised of 5 x Streptavidin polymer slides, 5 x Neutravidin polymer slides and 5 x 3D-Maleimid polymer slides. The package includes standard protocols for handling the different surfaces.
Image

More hydrophobic surfaces may result in reduced spot diameter, depending on the spotting buffer composition. Results may vary based on buffers, sample preparation, spotting and scanning instruments.

Please note, that in order to facilitate handling of the glass slides PolyAn also offers a range of useful accessories and reagents.

Selected Publications

  • Botti, V. et al., `Interaction between miR4749 and Human Serum Albumin as Revealed by Fluorescence, FRET, Atomic Force Spectroscopy and Computational Modelling´, Int J Mol Sci, 2022, 23, 1291. DOI: 10.3390/ijms23031291.
  • Heilkenbrinker, A. et al., `Identification of the Target Binding Site of Ethanolamine-Binding Aptamers and Its Exploitation for Ethanolamine Detection´, Anal Chem, 2015, 87, 677. DOI: 10.1021/ac5034819.
  • Kurth, T. et al., `Development of Aptamer-Based TID Assays Using Thermophoresis and Microarrays´, Biosensors, 2019, 9, 124. DOI: 10.3390/bios9040124.
  • Prante, M. et al., `Characterization of an Aptamer Directed against 25-Hydroxyvitamin D for the Development of a Competitive Aptamer-Based Assay´, Biosensors, 2019, 9, 134. DOI: 10.3390/bios9040134.
  • Wolff, N. et al., `Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA‑assays´, Sci Reports, 2021, 11, 6001. DOI: 10.1038/s41598-021-85438-5.
  • Wang, Z. et al., `Detection of genetic variation and base modifications at base-pair resolution on both DANN and RNA´, Commun Biology, 2021, 4, 128. DOI: 10.1038/s42003-021-01648-7.
  • Karadimas, D. et al., `LATE-PCR for LoC Molecular Diagnostics Devices and Ist Application to the Sensitive Detection of SARS-CoV-2´, Eng Proc, 2021, 6, 43. DOI: 10.3390/I3S2021Dresden-10076.
  • Díaz-Betancor, Z. et al., `Photoclick chemistry to create dextran-based nucleic acid microarrays´, Anal Bioanal Chem, 2019, 411, 6745. DOI: 10.1007/s00216-019-02050-3.
  • Sikora, K. et al., `A Universal Microarray Detection Method for Identification of Multiple Phytophthora spp. Using Padlock Probes´, Amer Phytopath Soc, 2012, 102, 635. DOI: 10.1094/PHYTO-11-11-0309.
  • Warmt, C. et al., `Investigation and validation of labelling loop mediated isothermal amplification (LAMP) products with different nucleotide modifications for various downstream analysis´, Sci Reports, 2022, 12, 7137. DOI: 10.1038/s41598-022-11320-7.
  • Warmt, C. et al., `Using Cy5‑dUTP labelling of RPA‑amplicons with downstream microarray analysis for the detection of antibiotic resistance genes´, Sci Reports, 2021, 11, 20137. DOI: 10.1038/s41598-021-99774-z.