Functionalized Coverslips

For high-resolution microscopic imaging or scanning applications PolyAn offers functionalized coverslips. All of PolyAn's standard functional coatings can also be applied to coverslip glass.
All coverslips have the standard dimension of 25 x 60mm. Type #1.5 is used as a standard thickness. All coverslips are packed in boxes of 5. Other functionalities and formats are available upon request. PolyAn also offers functionalised substrates that are coated with gold or other noble metals as well as waveguide materials.
2D-functionalized Coverslips
2D-Amino Coverslip, 5 pcs/box
104 00 026
2D-Amino functionalized Type #1.5 coverslips
2D-Carboxy Coverslip, 5 pcs/box
104 00 126
2D-Carboxy functionalized Type #1.5 coverslips
2D-Epoxy Coverslip, 5 pcs/box
104 00 226
2D-Epoxy functionalized Type #1.5 coverslips
2D-Aldehyde Coverslip, 5 pcs/box
104 00 326
2D-Aldehyde functionalized Type #1.5 coverslips
2D-Thiol Coverslip, 5 pcs/box
104 00 526
2D-Thiol functionalized Type #1.5 coverslips
2D-Azide Coverslip, 5 pcs/box
104 00 626
2D-Azide functionalized Type #1.5 coverslips. For covalent coupling of Alkyne-or DBCO modified biochemical species via click-chemistry.
For cost-sensitive applications PolyAn has developed a range of 2D (2-dimensional)-reactive coverslips that are manufactured from high quality glass with an ultra-flat surface and low inherent fluorescence. The glass is coated with a thin silane layer that will covalently bind most types of bio-molecules. The defect-free surface features uniform silane layers that provide a high covalent coupling efficiency together with a very low background.
3D-functionalized Coverslips
3D-Amino Coverslip, 5 pcs/box
104 00 006
3D-Amino functionalized Type #1.5 coverslips
3D-Carboxy Coverslip, 5 pcs/box
104 00 106
3D-Carboxy functionalized Type #1.5 coverslips
3D-Epoxy Coverslip, 5 pcs/box
104 00 206
3D-Epoxy functionalized Type #1.5 coverslips
3D-Aldehyde Coverslip, 5 pcs/box
104 00 306
3D-Aldehyde functionalized Type #1.5 coverslips
3D-NHS Coverslip, 5 pcs/box
104 00 407
3D-NHS functionalized Type #1.5 coverslips
3D-Activated Amino (PDITC) Coverslip, 5 pcs/box
104 00 436
3D-Activated Amino (PDITC) functionalized Type #1.5 coverslips
3D-Maleimide Coverslip, 5 pcs/box
104 00 446
3D-Maleimid functionalized Type #1.5 coverslips. Maleimide surfaces are used in „click chemistry“ for oriented covalent coupling (immobilization) of thiolated biochemical species.
3D-Thiol Coverslip, 5 pcs/box
104 00 507
3D-Thiol functionalized Type #1.5 coverslips
Covalently coated Poly-L-Lysin Coverslip, 5 pcs/box
104 01 206
Poly-L-Lysin functionalized Type #1.5 coverslips. Poly-L-Lysin surfaces are well suited for coupling of biochemical species. Especially, when the storage buffer contains glycerol Poly-L-Lysin surfaces are an interesting alternative to reactive matrices.
Covalently coated Streptavidin Coverslip, 5 pcs/box
104 02 206
Covalently coated Streptavidin, Type #1.5 coverslips
Covalently coated Neutravidin Coverslip, 5 pcs/box
104 03 206
Covalently coated Neutravidin, Type #1.5 coverslips
3D-Surfaces made by Molecular Surface Engineering

PolyAn’s 3D-functionalised microarray Slides are functionalized with a three-dimensional (3D)-surface chemistry comprised of a long-chain polymer containing a defined number of reactive groups. This polymer is covalently linked to the surface of the coverslip.
Our MSE-technology gently binds the functional layer onto the surface without damaging the base substrate. The morphology of the functional surface and thus the number of the reactive groups can be fine-tuned within a narrow range. This yields a number of advantages:
Low fluorescence background | Covalent binding of functional layer on the substrate without changing the initial autofluorescence. |
Low unspecific binding | Combination of reactive functional groups with PolyAn antifouling matrix. |
Topography | Tuneable surface hydrophilicity / hydrophobicity (contact angle). |
Uniform spot morphology | Narrow variation of surface properties and homogeneous distribution of reactive groups. |
Optimal density and high accessibility of reactive groups | Morphology and thickness of functional layer tailored to the desired application. |
Technical specifications
Packaging:
The coverslips are packaged in inert atmospheric boxes, with a capacity of 5 coverslips per box.
Storage
PolyAn’s coverslips are characterised by a long shelf-life when stored dry, at 4-8°C and protected from sunlight. All coverslips are packaged in boxes under Argon atmosphere to avoid contamination with particles. The Argon atmosphere also minimises degradation of the reactive surface through contact with air or humidity.