Functionalized Coverslips

For high-resolution microscopic imaging or scanning applications PolyAn offers functionalized coverslips. All of PolyAn's standard functional coatings can also be applied to coverslip glass.

All coverslips have the standard dimension of 25 x 60mm. Type #1.5 is used as a standard thickness. All coverslips are packed in boxes of 5. Other functionalities, thicknesses and sizes are available upon request. PolyAn also offers functionalised substrates that are coated with gold or other noble metals as well as waveguide materials.

2D-functionalized Coverslips

Id
Surface Modifications
Product Dimensions
Packaging Volume
Id Surface Modifications Product Dimensions Packaging Volume
104 00 026 2D-Amino 25 mm x 60 mm 5 coverslips/ Box
104 00 126 2D-Carboxy 25 mm x 60 mm 5 coverslips/ Box
104 00 226 2D-Epoxy 25 mm x 60 mm 5 coverslips/ Box
104 00 326 2D-Aldehyde 25 mm x 60 mm 5 coverslips/ Box
104 00 526 2D-Thiol 25 mm x 60 mm 5 coverslips/ Box
104 00 626 2D-Azide 25 mm x 60 mm 5 coverslips/ Box
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For cost-sensitive applications PolyAn has developed a range of 2D (2-dimensional)-reactive coverslips that are manufactured from high quality glass with an ultra-flat surface and low inherent fluorescence. The glass is coated with a thin silane layer that will covalently bind most types of bio-molecules. The defect-free surface features uniform silane layers that provide a high covalent coupling efficiency together with a very low background.

3D-functionalized Coverslips

Id
Surface Modifications
Product Dimensions
Packaging Volume
Id Surface Modifications Product Dimensions Packaging Volume
104 00 006 3D-Amino 25 mm x 60 mm 5 coverslips/ Box
104 00 106 3D-Carboxy 25 mm x 60 mm 5 coverslips/ Box
104 00 206 3D-Epoxy 25 mm x 60 mm 5 coverslips/ Box
104 00 306 3D-Aldehyde 25 mm x 60 mm 5 coverslips/ Box
104 00 407 3D-NHS 25 mm x 60 mm 5 coverslips/ Box
104 00 436 3D-Activated Amino (PDITC) 25 mm x 60 mm 5 coverslips/ Box
104 00 446 3D-Maleimide 25 mm x 60 mm 5 coverslips/ Box
104 00 507 3D-Thiol 25 mm x 60 mm 5 coverslips/ Box
104 01 206 Covalently coated Poly-L-Lysin 25 mm x 60 mm 5 coverslips/ Box
104 02 206 Covalently coated Streptavidin 25 mm x 60 mm 5 coverslips/ Box
104 03 206 Covalently coated Neutravidin 25 mm x 60 mm 5 coverslips/ Box
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3D-Surfaces made by Molecular Surface Engineering

PolyAn’s 3D-functionalised microarray Slides are functionalized with a three-dimensional (3D)-surface chemistry comprised of a long-chain polymer containing a defined number of reactive groups. This polymer is covalently linked to the surface of the coverslip.

Our MSE-technology gently binds the functional layer onto the surface without damaging the base substrate. The morphology of the functional surface and thus the number of the reactive groups can be fine-tuned within a narrow range. This yields a number of advantages:

 

Low fluorescence background Covalent binding of functional layer on the substrate without changing the initial autofluorescence.
Low unspecific binding Combination of reactive functional groups with PolyAn antifouling matrix.
Topography Tuneable surface
hydrophilicity / hydrophobicity (contact angle).
Uniform spot morphology Narrow variation of surface properties and homogeneous distribution of reactive groups.
Optimal density and high accessibility of reactive groups Morphology and thickness of functional layer tailored to the desired application.

 

Technical specifications

Packaging:

The coverslips are packaged in inert atmospheric boxes, with a capacity of 5 coverslips per box.

Storage

PolyAn’s coverslips are characterised by a long shelf-life when stored dry, at 4-8°C and protected from sunlight. All coverslips are packaged in boxes under Argon atmosphere to avoid contamination with particles. The Argon atmosphere also minimises degradation of the reactive surface through contact with air or humidity.