Functionalized Coverslips

For high-resolution microscopic imaging or scanning applications PolyAn offers functionalized coverslips. All of PolyAn's standard functional coatings can also be applied to coverslip glass.

All coverslips have the standard dimension of 25 x 60mm. Type #1.5 is used as a standard thickness. All coverslips are packed in boxes of 5. Other functionalities and formats are available upon request. PolyAn also offers functionalised substrates that are coated with gold or other noble metals as well as waveguide materials.

2D-functionalized Coverslips

IdSurface ModificationsProduct DimensionsPackaging Volume
104 00 0262D-Amino25 mm x 60 mm5 coverslips/ BoxInfo
104 00 2262D-Epoxy25 mm x 60 mm5 coverslips/ BoxInfo
104 00 3262D-Aldehyde25 mm x 60 mm5 coverslips/ BoxInfo
104 00 5262D-Thiol25 mm x 60 mm5 coverslips/ BoxInfo
104 00 6262D-Azide25 mm x 60 mm5 coverslips/ BoxInfo
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For cost-sensitive applications PolyAn has developed a range of 2D (2-dimensional)-reactive coverslips that are manufactured from high quality glass with an ultra-flat surface and low inherent fluorescence. The glass is coated with a thin silane layer that will covalently bind most types of bio-molecules. The defect-free surface features uniform silane layers that provide a high covalent coupling efficiency together with a very low background.

3D-functionalized Coverslips

IdSurface ModificationsProduct DimensionsPackaging Volume
104 00 0063D-Amino25 mm x 60 mm5 coverslips/ BoxInfo
104 00 0123D-Epoxy25 mm x 60 mm5 coverslips/ BoxInfo
104 00 1063D-Carboxy25 mm x 60 mm5 coverslips/ BoxInfo
104 00 3063D-Aldehyde25 mm x 60 mm5 coverslips/ BoxInfo
104 00 4073D-NHS25 mm x 60 mm5 coverslips/ BoxInfo
104 00 4363D-Activated Amino (PDITC)25 mm x 60 mm5 coverslips/ BoxInfo
104 00 4463D-Maleimide25 mm x 60 mm5 coverslips/ BoxInfo
104 01 206Covalently coated Poly-L-Lysin25 mm x 60 mm5 coverslips/ BoxInfo
104 02 206Covalently coated Streptavidin25 mm x 60 mm5 coverslips/ BoxInfo
104 03 206Covalently coated Neutravidin25 mm x 60 mm5 coverslips/ BoxInfo
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3D-Surfaces made by Molecular Surface Engineering

PolyAn’s 3D-functionalised microarray Slides are functionalized with a three-dimensional (3D)-surface chemistry comprised of a long-chain polymer containing a defined number of reactive groups. This polymer is covalently linked to the surface of the coverslip.

Our MSE-technology gently binds the functional layer onto the surface without damaging the base substrate. The morphology of the functional surface and thus the number of the reactive groups can be fine-tuned within a narrow range. This yields a number of advantages:

 

Low fluorescence backgroundCovalent binding of functional layer on the substrate without changing the initial autofluorescence.
Low unspecific bindingCombination of reactive functional groups with PolyAn antifouling matrix.
TopographyTuneable surface
hydrophilicity / hydrophobicity (contact angle).
Uniform spot morphologyNarrow variation of surface properties and homogeneous distribution of reactive groups.
Optimal density and high accessibility of reactive groupsMorphology and thickness of functional layer tailored to the desired application.

Technical specifications

Packaging:

The coverslips are packaged in inert atmospheric boxes, with a capacity of 5 coverslips per box.

Storage

PolyAn’s coverslips are characterised by a long shelf-life when stored dry, at room temperature and protected from sunlight. All coverslips are packaged in boxes under Argon atmosphere to avoid contamination with particles. The Argon atmosphere also minimises degradation of the reactive surface through contact with air or humidity.