Spectrum Calibration Nanobeads

PolyAn’s Spectrum Calibration Nanobeads can help adjust fluorescence-based detection systems such as flow cytometers, conventional fluorescence microscopes, confocal laser scanning microscopes, super resolution microscopes, and other image-processing systems.

PolyAn’s Spectrum Calibration Nanobeads are particularly useful to calibrate imaging setups for multi-color applications and to check their capability to resolve and co-localize very small fluorescent objects of different color in the same optical plane.

As Spectrum Calibration Nanobeads resemble the size of extracellular vesicles (EVs), they can also be used to adjust flow cytometers or other imaging platforms for EV detection. PMMA-Nanobeads compare favorably with polystyrene particles due to their lower refractive index (RI), which is closer to the RI of EVs.

125 nm Spectrum Calibration Nanobeads

Products

Id
Title
Solids Content
Packaging Volume
Id Title Solids Content Packaging Volume
208 02 125 100-150nm, Carboxy, Spectrum Calibration Nanobeads, 1 population 0.1% 1.5 mL
208 02 500 551-600nm, Carboxy, Spectrum Calibration Nanobeads, 1 population 0.1% 1.5 mL
208 03 125 100-150nm, Aldehyde, Spectrum Calibration Nanobeads, 1 population 0.1% 1.5 mL
208 03 500 551-600nm, Aldehyde, Spectrum Calibration Nanobeads, 1 population 0.1% 1.5 mL
208 04 125 100-150nm, Streptavidin, Spectrum Calibration Nanobeads, 1 population 0.1% 1.5 mL
208 04 500 551-600nm, Streptavidin, Spectrum Calibration Nanobeads, 1 population 0.1% 1.5 mL
208 05 125 100-150nm, Neutravidin, Spectrum Calibration Nanobeads, 1 population 0.1% 1.5 mL
208 05 500 551-600nm, Neutravidin, Spectrum Calibration Nanobeads, 1 population 0.1% 1.5 mL
208 12 125 100-150 nm, Carboxy, Spectrum Calibration Nanobeads, 4 populations (peaks) 0.1% 1.5 mL
208 12 500 475-525 nm, Carboxy, Spectrum Calibration Nanobeads, 4 populations (peaks) 0.1% 1.5 mL
208 13 125 100-150 nm, Aldehyde, Spectrum Calibration Nanobeads, 4 populations (peaks) 0.1% 1.5 mL
208 13 500 475-525 nm, Aldehyde, Spectrum Calibration Nanobeads, 4 populations (peaks) 0.1% 1.5 mL
208 14 125 100-150 nm, Streptavidin, Spectrum Calibration Nanobeads, 4 populations (peaks) 0.1% 1.5 mL
208 14 500 475-525 nm, Streptavidin, Spectrum Calibration Nanobeads, 4 populations (peaks) 0.1% 1.5 mL
208 15 125 100-150 nm, Neutravidin, Spectrum Calibration Nanobeads, 4 populations (peaks) 0.1% 1.5 mL
208 15 500 475-525 nm, Neutravidin, Spectrum Calibration Nanobeads, 4 populations (peaks) 0.1% 1.5 mL
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Key features

PolyAn's Spectrum Calibration Nanobeads are monodisperse PMMA sub-micron particles that are available in different sizes with diameters between 100 nm and 600 nm. The beads contain a mixture of highly photostable fluorescent dyes that are homogeneously encapsulated within the PMMA matrix.

The Spectrum Calibration Nanobeads can be excited at any wavelength from 365–650 nm and emit at 400–750 nm, which enables their detection in all common fluorescence detection channels (DAPI, FITC, PE/TAMRA/Cy3®, APC/Cy5®, Cy7®) with only minimal photobleaching.

Spectrum Calibration Nanobeads with 125 nm (top) and 500 nm (bottom) particle size measured in the FITC and APC detection channel of a CytoFLEX nano (Beckman Coulter Life Sciences) flow cytometer.*

Spectrum Calibration Nanobeads are available in all sizes from 100–600 nm upon request. Please enquire regarding different fluorescence intensities and other surface functionalities.

PolyAn also offers Spectrum Calibration Microbeads with sizes ranging from 2–20 µm. Please do not hesitate to contact our customer service for a custom development.

 

[*Courtesy of Dr. Alfonso Blanco Fernández, Flow Cytometry Core Facility Director at University College Dublin, Conway Institute of Biomolecular & Biomedical Research]