3D-Epoxy Microplates
for covalent coupling of biomolucles via nucleophilic groups (e.g. amine groups)
Epoxides are cyclic ethers with a highly strained three ring. Epoxy rings can be easily reacted with nucleophils e.g. amines, hydrazines, thiols, hydroxides and carboxyl groups. Compared to 3D-NHS matrices the epoxy surface is more stable and has a longer shelf-life. Epoxy-surfaces are stable up temperatures of 40°C and are also more stable against humidity compared to NHS-surfaces.
Products
Key Features
- For covalent immobilization of biomolecules
- Reacts with nucleophilic groups, e.g. amines, thioles, and hydroxyl groups
- Uncharged surface with integrated low fouling matrix
- Long shelf-life of up to two years
The 3D-Epoxy Microplates are used mainly if adsorptive binding of peptides, oligonucleotides or proteins, for example, to high/medium binding surfaces is ineffective or the binding strength is not sufficient. Epoxy rings can easily react with nucleophiles e.g. amines, hydrazines, thiols, hydroxides and carboxyl groups of biomolecules to form a covalent bond with the surface.
The nucleophilic addition is catalysed by acid or basic conditions. Under acidic conditions, the oxygen in the ring is positively charged, which facilitates the nucleophilic attack. Under basic conditions the least substituted carbon is attacked by the applied nucleophil in a standard SN2 reaction.
PolyAn also equips glass slides, coverslips, polymer slides, and COP films with 3D-Epoxy surfaces. Please do not hesitate to contact us, if you would like to functionalize a different format or substrate with our 3D-Epoxy surface.
3D-Epoxy surface for ELISA applications
Comparison of IgG-FITC binding onto PolyAn's 3D-Epoxy surface versus passive (adsorptive) immobilization
Selected Publications
- Tuncay, A. et al., `Performance benchmarking microplate-immunoassays for quantifying target-specific cysteine oxidation reveals their potential for understanding redox-regulation and oxidative stress´, Free Rad. Biol. Med., 2023, 204, 252. DOI: 10.1016/j.freeradbiomed.2023.05.006.
- Tuncay, A. et al., `RedoxiFluor: A microplate technique to quantify target-specific protein thiol redox state in relative percentage and molar terms´, Free Rad. Biol. Med., 2022, 181, 118. DOI: 10.1016/j.freeradbiomed.2022.01.023.
- Noble, A. et al., `ALISA: A microplate assay to measure protein thiol redox state´, Free Rad. Biol. Med., 2021, 174, 272. DOI: 10.1016/j.freeradbiomed.2021.08.018.