PolyAn offers functionalized Nanobeads and small Microparticles for agglutination assays.
Nanobead-based agglutination assay for the discrimination between different E. coli serotypes: 130 nm Nanobeads were coupled to a goat-antibody recognizing E. coli serotype O26. Subsequently, the Nanobead suspension was mixed with E. coli suspension containing serotype O157 (negative control) or O26. The suspensions were placed between a microscope slide and a cover slip. After a 5 min incubation, the suspension was analyzed with a fluorescence microscope at 488 nm.
To verify the presence of antibodies on the Nanobead surface, the Nanobeads were mixed with Protein G-coupled microparticles. The binding of the fluorescently labelled Nanobeads on the microparticles was detected using fluorescence microscopy:
Liebsch, C. et al., `Solid-phase microbead array for multiplex O-serotyping of Escherichia coli´, Microchim. Acta, 2017, 184, 1405. DOI: 10.1007/s00604-017-2088-4.