Aldehyde Surfaces

for coupling via the N-terminus of the biochemical species

Aldehyde groups react immediately with the NH₂-terminus of biochemical species to form a covalent bond with the surface (420 kJ/mol). In an intermediate state the Aldehydes form an instable Imine-group with the Amines (Schiff-base). It is also possible to reduce the Imines with Sodium Borhydride to form stable Amines.

Upon completion the coupling reaction other non-reacted aldehydes must be blocked with small molecules that penetrate the 3D-Matrix and effectively quench all remaining reactive groups.

PolyAn equips glass slides, coverslips and polymer slides with 3D-Aldehyde surfaces. Please do not hesitate to contact us, if you would like to functionalize a different format or substrate with our 3D-Aldehyde surface.

Selected Publications

Aldehyde surface for the immobilization of Proteins and Antibodies:

Lussier, F. et al., `PiP-plex: A Particle-in-Particle System for Multiplexed Quantification of Secreted Proteins by Single Cells´, bioRxiv, 2025, DOI: 10.1101/2025.03.26.645608.
Pancrazi, F. et al., `Proline isomerization modulates the bacterial IsdB/hemoglobin interaction: an atomic force spectroscopy study´, Discover Nano, 2025, 20, 20. DOI: 10.1186/s11671-025-04182-1.
Botti, V. et al., `Nanoscale dynamical investigation of the hemoglobin complex with the bacterial protein IsdB: is their interaction stabilized by catch bonds?´, Nanoscale, 2024, 16, 4308. DOI: 10.1039/d3nr05241a.
Shen, M.L. et al., `Protein Co-Enrichment Analysis of Extracellular Vesicles´, arXiv, 2023, DOI: 10.48550/arXiv.2301.04051.
Martel, R. et al., `Extracellular Vesicle Antibody Microarray for Multiplexed Inner and Outer Protein Analysis´, ACS Sensors, 2022, 7, 3817. DOI: 10.1021/acssensors.2c01750.
Horta, S. et al., `Evaluation of Immuno-Rolling Circle Amplification for Multiplex Detection and Profiling of Antigen-Specific Antibody Isotypes´, Anal. Chem., 2021, 93, 6169. DOI: 10.1021/acs.analchem.1c00172.
Normandeau, F. et al., `Spatial Bias in Antibody Microarrays May Be an Underappreciated Source of Variability´, ACS Sensors, 2021, 6, 1796. DOI: 10.1021/acssensors.0c02613.
Goyette, A.-P. et al., `Microfluidic multipoles theory and applications´, Nature Commun., 2019, 10, 1781. DOI: 10.1038/s41467-019-09740-7.
Laforte, V. et al., `Antibody Colocalization Microarray for Cross-Reactivity-Free Multiplexed Protein Analysis´, Meth. Mol. Biol., 2017, 1619, 239. DOI: 10.1007/978-1-4939-7057-5_19,.
Moscetti, I. et al., `Binding kinetics of mutant p53R175H with wild type p53 and p63: A Surface Plasmon Resonance and Atomic Force Spectroscopy study´, Biophys. Chem., 2017, 228, 55. DOI: 10.1016/j.bpc.2017.07.002.

Aldehyde surface for the immobilization of Oligonucleotides (DNA/RNA):

Botti, V. et al., `Interaction between miR4749 and Human Serum Albumin as Revealed by Fluorescence, FRET, Atomic Force Spectroscopy and Computational Modelling´, Int. J. Mol. Sci., 2022, 23, 1291. DOI: 10.3390/ijms23031291.
Wolff, N. et al., `Full pathogen characterisation: species identification including the detection of virulence factors and antibiotic resistance genes via multiplex DNA‑assays´, Sci. Rep., 2021, 11, 6001. DOI: 10.1038/s41598-021-85438-5.
Kurth, T. et al., `Development of Aptamer-Based TID Assays Using Thermophoresis and Microarrays´, Biosensors, 2019, 9, 124. DOI: 10.3390/bios9040124.
Prante, M. et al., `Characterization of an Aptamer Directed against 25-Hydroxyvitamin D for the Development of a Competitive Aptamer-Based Assay´, Biosensors, 2019, 9, 134. DOI: 10.3390/bios9040134.
Heilkenbrinker, A. et al., `Identification of the Target Binding Site of Ethanolamine-Binding Aptamers and Its Exploitation for Ethanolamine Detection´, Anal. Chem., 2015, 87, 677. DOI: 10.1021/ac5034819.

Aldehyde surface for the immobilization of Peptides:

Rapsch, K. et al., `Identification of antimicrobial peptides and immobilization strategy suitable for a covalent surface coating with biocompatible properties´, Bioconjugate Chem., 2014, 25, 308. DOI: 10.1021/bc4004469.

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