for oriented covalent coupling (immobilization) of thiolated biochemical species
Maleimide-esters react immediately with Thiol-groups of biochemical species. The Thiol-groups can be either natively present in the (bio)molecule, e.g. through the amino acid cysteine in proteins, produced via reductive cleavage of disulfide bonds with a reducing agent such as Dithiothreitol (DTT, Cleland´s Reagent), or selectively introduced e.g. with 2-Iminothiolane (Traut's reagent) for amine-containing molecules.
Similar to NHS-esters, Maleimide surfaces are susceptible to hydrolysis, and thus, should be processed promptly after opening the sealed bags.
PolyAn equips glass slides, coverslips and polymer slides with 3D-Maleimide surfaces. Please do not hesitate to contact us, if you would like to functionalize a different format or substrate with our 3D-Maleimide surface.
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