for oriented covalent coupling (immobilization) of thiolated biochemical species
Maleimide-esters react immediately with Thiol-groups of biochemical species. The Thiol-groups can be either natively present in the (bio)molecule, e.g. through the amino acid cysteine in proteins, produced via reductive cleavage of disulfide bonds with a reducing agent such as Dithiothreitol (DTT, Cleland´s Reagent), or selectively introduced e.g. with 2-Iminothiolane (Traut's reagent) for amine-containing molecules.
Similar to NHS-esters, Maleimide surfaces are susceptible to hydrolysis, and thus, should be processed promptly after opening the sealed bags.
PolyAn equips glass slides, coverslips and polymer slides with 3D-Maleimide surfaces. Please do not hesitate to contact us, if you would like to functionalize a different format or substrate with our 3D-Maleimide surface.
- Broecker, F. et al., `Synthetic Glycan Microarrays´, Meth Mol Biol, 2017, 1518, 227. DOI: 10.1007/978-1-4939-6584-7_15.
- Götze, S. et al., `Investigation of the protective properties of glycosylphosphatidylinositol-based vaccine candidates in a Toxoplasma gondii mouse challenge model´, Glycobiology, 2015, 25, 984. DOI: 10.1093/glycob/cwv040.
- Malik, A. et al., `Immunological Evaluation of Synthetic Glycosylphosphatidylinositol Glycoconjugates as Vaccine Candidates against Malaria´, Chem Biol, 2020, 15, 171. DOI: 10.1021/acschembio.9b00739.