for oriented covalent coupling (immobilization) of thiolated biochemical species
This covalent coupling method is suitable for the oriented or othogonal coupling of thiol modified probes or biochemical species. Only thiol groups can be added on the double bond of the Maleimide active esters. Similar to the NHS- modification the formed esters are susceptible against hydrolysis. Thus, we recommend processing the slides immediately after opening the sealed bags. Thiolated probes or biochemical species containing disulfide bridged dimers must be reduced e. g. with Dithiothreitol (DTT) or Cleland´s reagent, for an optimal and effective surface coupling.
PolyAn equips glass slides and polymer slides with 3D-Maleimide surfaces. Please do not hesitate to contact us, if you would like to functionalize a different format or substrate with our 3D-Maleimide surface.