Streptavidin- and Neutravidin-Microplates

PolyAn is offering Streptavidin-/Neutravidin-coated microplates that are suitable for immunoassays, DNA hybridization assays, and other demanding bioassays that utilize biotinylated moieties. The Streptavidin- and Neutravidin-coated plates are made using PolyAn’s proprietary Molecular Surface Engineering technology to achieve maximum efficient coating with Streptavidin or Neutravidin.

Key features

  • No leaching of Streptavidin and Neutravidin due to covalent immobilization
  • Minimal unspecific binding due to 3D-functional matrix
  • Ready-to-use: no additional washing or blocking steps required
  • Available for colorimetric, chemiluminescence, or fluorescent detection
  • Each plate is packaged in a separate foil bag to ensure optimal shelf-life

Products

Id
Surface Modifications
Format
Id Surface Modifications Format
00 681 252 Streptavidin (covalently coated) 96 well plate, white, 12 x 8-strip, flat bottom
00 682 252 Neutravidin (covalently coated) 96 well plate, white, 12 x 8-strip, flat bottom
00 692 252 Streptavidin (covalently coated) 96 well plate, transparent, 12 x 8-strip, flat bottom
00 693 252 Neutravidin (covalently coated) 96 well plate, transparent, 12 x 8-strip, flat bottom
00 697 451 Streptavidin (covalently coated) 96 well plate ELISA, C-bottom, transparent, 12 x 8 strip
00 698 451 Neutravidin (covalently coated) 96 well plate ELISA, C-bottom, transparent, 12 x 8 strip
00 700 501 Streptavidin (covalently coated) 96 Round MultiWell Plate, Black Polystyrene, Glass Bottom
00 700 551 Neutravidin (covalently coated) 96 Round MultiWell Plate, Black Polystyrene, Glass Bottom
00 705 501 Streptavidin (covalently coated) 96 Square MultiWell Plate, Black Polystyrene, Glass Bottom
00 705 551 Neutravidin (covalently coated) 96 Square MultiWell Plate, Black Polystyrene, Glass Bottom
00 710 501 Streptavidin (covalently coated) 384 Square MultiWell Plate, Black Polystyrene, Glass Bottom
00 710 551 Neutravidin (covalently coated) 384 Square MultiWell Plate, Black Polystyrene, Glass Bottom
00 791 252 Streptavidin (covalently coated) 96-Well µClear® plate, polystyrene, black
00 792 252 Neutravidin (covalently coated) 96-Well µClear® plate, polystyrene, black
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Additionally, PolyAn offers a range of glass bottom plates in a wide range of surfaces and well designs. PolyAn also equips glass slides and polymer slides with Streptavidin and Neutravidin surfaces as well as solvent stable microplates that are comprised of Polypropylene (PP).

Benefits of covalent coating

PolyAn’s Streptavidin or Neutravidin matrices are covalently attached to the surface, so that the molecules are less susceptible to desorption in the presence of surfactants, solutions of high ionic strength, or at high temperatures, compared to adsorptive immobilization.

Advantages of Neutravidin

Neutravidin is a de-glycosylated form of avidin. Although Streptavidin is still the most popular and widely used biotin-binding protein, Neutravidin offers several benefits:

  • Near-neutral isoelectric point (pI) of 6.3 efficiently decreases non-specific interactions
  • De-glycosylation (carbohydrate removal) prevents non-specific binding of lectins
  • Lacks the bacterial RYD sequence (Arg-Tyr-Asp) of SAv that causes non-specific binding in cell assays

Neutravidin's high biotin-binding affinity and low non-specific binding make it the ideal biotin-binding protein!

Customized plate surfaces

Please do not hesitate to contact us, if you require a special surface for binding of your biomolecules that is not listed in the products table. Other plate formats and substrates can be equipped with our surfaces as part of our Molecular Surface Engineering Services. We are also happy to support you in developing suitable protocols for coating on our plates.