Microarray Slides for glycan (carbohydrate) applications
Glycan microarrays are presentations of multiple glycans or glycoconjugates printed on a slide for screening with glycan-binding proteins, e.g. lectins, antibodies, bacteria, and viruses.
All of PolyAn’s reactive surfaces are completely transparent. They are characterised by a low lot-to-lot variation that is specified and monitored by using contact angle measurements as well as qualitative test methods.
Glycans are usually derivatized with functional groups and can be immobilzed for example on PolyAn's 2D-Epoxy, 3D-Epoxy and 3D-NHS functionalized glass slides or onto PolyAn's functionalized 96-well plates.
A flyer showing the most commonly used functionalized surfaces for Glycan Microarrays available from PolyAn can be downloaded here.
More hydrophobic surfaces may result in reduced spot diameter, depending on the spotting buffer composition. Results may vary based on buffers, sample preparation, spotting and scanning instruments.
Please note, that in order to facilitate handling of the glass slides PolyAn also offers a range of useful accessories and reagents.
Selected Publications
- Khan, N. et al., `Sialoglycan-binding patterns of bacterial AB5 toxin B subunits correlate with host range and toxicity, indicating evolution independent of A subunits´, J. Biol. Chem. 2022, 298, 101900. DOI: 10.1016/j.jbc.2022.101900.
- Sasmal, A. et al., `Simple and practical sialoglycan encoding system reveals vast diversity in nature and identifies a universal sialoglycan-recognizing probe derived from AB5 toxin B subunits´, Glycobiology 2022, 32, 1101. DOI: 10.1093/glycob/cwac057.
- Ji, Y. et al., `Reversible O‑Acetyl Migration within the Sialic Acid Side Chain and Its Influence on Protein Recognition´, Chem. Biol. 2021, 16, 1951. DOI: 10.1021/acschembio.0c00998.
- Noy-Porat, T. et al., `Therapeutic antibodies, targeting the SARS-CoV-2 spike N-terminal domain, protect lethally infected K18-hACE2 mice´, iScience 2021, 24, 102479. DOI: 10.1016/j.isci.2021.102479.
- Saha, S. et al., `Exploring the Impact of Ketodeoxynonulosonic Acid in Host-Pathogen Interactions Using Uptake and Surface Display by Nontypeable Haemophilus influenzae´, mBio 2021, 12, 03226-20. DOI: 10.1128/mBio.03226-20.
- Shanthamurthy, C.D. et al., `Heparan Sulfate Mimetics Differentially Affect Homologous Chemokines and Attenuate Cancer Development´, J. Med. Chem. 2021, 64, 3367. DOI: 10.1021/acs.jmedchem.0c01800.
- Siddiqui, S.S. et al., `Sialoglycan recognition is a common connection linking acidosis, zinc, and HMGB1 in sepsis´, PNAS 2021, 118, 2018090118. DOI: 10.1073/pnas.2018090118.
- Tsouka, A. et al., `Probing Multivalent Carbohydrate-Protein Interactions With On-Chip Synthesized Glycopeptides Using Different Functionalized Surfaces´, Front. Chem. 2021, 9, 766932. DOI: 10.3389/fchem.2021.766932.
- Bashir, S. et al., `Association between Neu5Gc carbohydrate and serum antibodies against it provides the molecular link to cancer: French NutriNet-Santé study´, BMC Medicine 2020, 18, 262. DOI: 10.1186/s12916-020-01721-8.
- Malik, A. et al., `Immunological Evaluation of Synthetic Glycosylphosphatidylinositol Glycoconjugates as Vaccine Candidates against Malaria´, Chem. Biol. 2020, 15, 171. DOI: 10.1021/acschembio.9b00739.
- Ruprecht, C. et al., `Practical considerations for printing high-density glycan microarrays to study weak carbohydrate-protein interactions´, Carbohydrate Res. 2019, 481, 31. DOI: 10.1016/j.carres.2019.06.006.
- Gade, M. et al., `Microarray Analysis of Oligosaccharide-Mediated Multivalent Carbohydrate–Protein Interactions and Their Heterogeneity´, ChemBioChem 2018, 19, 1170. DOI: 10.1002/cbic.201800037.
- Shanthamurthy, C.D. et al., `ABO Antigens Active Tri- and Disaccharides Microarray to Evaluate C-type Lectin Receptor Binding Preferences´, Sci. Reports 2018, 8, 6603. DOI: 10.1038/s41598-018-24333-y.
- Ben-Arye, S.L. et al., `Profiling Anti-Neu5Gc IgG in Human Sera with a Sialoglycan Microarray Assay´, J. Vis. Exp. 2017, 125, 56094. DOI: 10.3791/56094.
- Broecker, F. et al., `Synthetic Glycan Microarrays´, Meth. Mol. Biol. 2017, 1518, 227. DOI: 10.1007/978-1-4939-6584-7_15.
- Götze, S. et al., `Investigation of the protective properties of glycosylphosphatidylinositol-based vaccine candidates in a Toxoplasma gondii mouse challenge model´, Glycobiology 2015, 25, 984. DOI: 10.1093/glycob/cwv040.