Differences in fluorescence-lifetime are for example used in fluorescence lifetime imaging microscopy (FLIM). FLIM is an imaging technique for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample. It is used in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography.
Our PolyAn FLT beads are also available with Streptavidin, Neutravidin, Protein A/G, 3D-Azide and 3D-Alkyne surfaces. A custom modification with antibodies, peptides or oligonucleotides is available upon request.
- D. Kage, L. Fischer, K. Hoffmann, T. Thiele, U. Schedler, and U. Resch-Genger: Close Spectroscopic Look at Dye-Stained Polymer Microbeads. Journal of Physical Chemistry C 122, 12782-12791, 2018.
- D. Kage, K. Hoffmann, M. Wittkamp, J. Ameskamp, W. Göhde, and U. Resch-Genger: Luminescence lifetime encoding in time-domain flow cytometry. Scientific Reports (2018) 8:16715 | DOI:10.1038/s41598-018-35137-5 1.