Microparticles for Fluorescence Lifetime Applications

Differences in fluorescence-lifetime are for example used in fluorescence lifetime imaging microscopy (FLIM). FLIM is an imaging technique for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample. It is used in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography.

Products

Id
Title
Id Title
110 00 006 6.5 µm, 3D-Carboxy Fluorescence Lifetime Beads, 1.7 ns
110 10 006 6.5 µm, 3D-Carboxy Fluorescence Lifetime Beads, 2.7 ns
110 20 006 6.5 µm, 3D-Carboxy Fluorescence Lifetime Beads, 5.5 ns
110 30 006 6.5 µm, 3D-Carboxy Fluorescence Lifetime Beads, 7.9 ns
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The standard packaging volume is 1.5 mL with a solids content of 0.1% (mg/mL). Our PolyAn FLT beads are also available with Streptavidin, Neutravidin, Protein A/G, 3D-Azide and 3D-Alkyne surfaces. A custom modification with antibodies, peptides or oligonucleotides is available upon request.

Publications

  • D. Kage, K. Hoffmann, H. Borcherding, U. Schedler, U. Resch-Genger: Lifetime encoding in flow cytometry for bead-based sensing of biomolecular interaction, 2020, Scientific Reports 10, 19477.
  • D.Kage, K. Hoffmann, G. Nifontova, V. Krivenkov, A. Sukhanova, I. Nabiev, U. Resch-Genger: Tempo-spectral multiplexing in flow cytometry with lifetime detection using QD-encoded polymer beads, 2020, Scientific Reports 10, 653.
  • D. Kage, L. Fischer, K. Hoffmann, T. Thiele, U. Schedler, and U. Resch-Genger: Close spectroscopic look at dye-stained polymer microbeads, 2018, Journal of Physical Chemistry C 122, 12782-12791.
  • D. Kage, K. Hoffmann, M. Wittkamp, J. Ameskamp, W. Göhde, and U. Resch-Genger: Luminescence lifetime encoding in time-domain flow cytometry, 2018, Scientific Reports 8, 16715.