Differences in fluorescence-lifetime are for example used in fluorescence lifetime imaging microscopy (FLIM). FLIM is an imaging technique for producing an image based on the differences in the exponential decay rate of the fluorescence from a fluorescent sample. It is used in confocal microscopy, two-photon excitation microscopy, and multiphoton tomography.
6.5 µm, 3D-Carboxy Fluorescence Lifetime Beads, 1.7 ns
110 00 006
6.5 µm, 3D-Carboxy Fluorescence Lifetime Beads, 2.7 ns
110 10 006
6.5 µm, 3D-Carboxy Fluorescence Lifetime Beads, 5.5 ns
110 20 006
6.5 µm, 3D-Carboxy Fluorescence Lifetime Beads, 7.9 ns
110 30 006
The standard packaging volume is 1.5 mL with a solids content of 0.1% (mg/mL). Our PolyAn FLT beads are also available with Streptavidin, Neutravidin, Protein A/G, 3D-Azide and 3D-Alkyne surfaces. A custom modification with antibodies, peptides or oligonucleotides is available upon request.
- D. Kage, L. Fischer, K. Hoffmann, T. Thiele, U. Schedler, and U. Resch-Genger: Close Spectroscopic Look at Dye-Stained Polymer Microbeads. Journal of Physical Chemistry C 122, 12782-12791, 2018.
- D. Kage, K. Hoffmann, M. Wittkamp, J. Ameskamp, W. Göhde, and U. Resch-Genger: Luminescence lifetime encoding in time-domain flow cytometry. Scientific Reports (2018) 8:16715 | DOI:10.1038/s41598-018-35137-5 1.