Fluorescence lifetime (FLT) measurements can be used to discriminate fluorescent samples based on differences in their fluorescence decay rates. This approach can be applied for fluorescence lifetime imaging microscopy (FLIM), confocal microscopy, two-photon excitation microscopy, multiphoton tomography as well as for lifetime-based flow cytometry (FCM). PolyAn has developed fluorescence lifetime encoded beads (FLT beads) that can be distinguished according to their different fluorescence lifetimes.
The standard packaging volume is 1.5 mL with a solids content of 0.1% (mg/mL).
Our PolyAn FLT Beads are also available with 3D-Aldehyde, Streptavidin or Neutravidin, Protein A/G, 3D-Azide, 3D-Alkyne, 3D-DBCO, and 3D-MTZ surfaces. A custom modification with antibodies, peptides, or oligonucleotides is available upon request.
- Kage, D. et al., `Tempo-spectral multiplexing in flow cytometry with lifetime detection using QD-encoded polymer beads´, Sci. Rep., 2020, 10, 653. DOI: 10.1038/s41598-019-56938-2.
- Kage, D. et al., `Lifetime encoding in flow cytometry for bead‑based sensing of biomolecular interaction´, Sci. Rep., 2020, 10, 19477. DOI: 10.1038/s41598-020-76150-x.
- Kage, D. et al., `Luminescence lifetime encoding in time-domain flow cytometry´, Sci. Rep., 2018, 8, 16715. DOI: 10.1038/s41598-018-35137.
* Images courtesy of Bundesanstalt für Materialforschung und -prüfung (BAM), D. Kage et al., Luminescence lifetime encoding in time-domain flow cytometry.
Scientific Reports (2018) 8: Article 16715.