Nitrocellulose Film Slides

PolyAn is the European distributor of the nitrocellulose based microarray product families developed by Grace Bio-Labs.

ONCYTE® porous nitrocellulose (PNC) slides are the optimal high binding protein microarray substrate technology available. Invented by Grace Bio-Labs scientists, the three dimensional microporous film cast on a variety of solid surfaces (vis. glass, plastic, gold, tantalum) is comprised of a nitrocellulose polymer and proprietary chemistry that retains stable protein quaternary conformations providing ideal microarray substrate for a variety of applications.  ONCYTE® film provides an excellent surface when performing reverse phase protein arrays (RPPA), antibody arrays, antigen or peptide arrays whether using colorimetric,fluorescent or near infra-red detection systems.

 

AVID

NOVA

SuperNOVA

PATH

Binding Capacity ++++ ++ ++++ +
Fluorescence background + ++ +++ ++++
Dynamic range (log scale fluorescence) 5-6 5-6 7+ 4-5
Hydrophobicity    + + + ++
Applications Best for any application requiring high binding capacity and colorimetric detection. Reduced fluorescence background with lower binding capacity than AVID. Good signal-to-noise ratio for fluorescence detection. Second generation NOVA, lowest fluorescence background, high binding capacity. Best for fluorescence detection and large dynamic range. Lowest fluorescence background, lower binding capacity, reduced dynamic range. Best signal-to-noise ratio for fluorescence detection.

 

The ONCYTE™ and SuperNOVA™ slides are available as single film or in various multi-pad formats. The film is compatible with fluorescent, chemoluminescent, radiographic, and colorimetric detection systems. It is manufactured to the highest standards, providing excellent reliability and reproducibility.

The PATH® Protein Microarray Slide is coated with an ultra-thin nitrocellulose film for the noncovalent, yet irreversible, binding of printed antibodies to the slide surface while maintaining antigen binding capability. PATH® slides provide maximum reliability, sensitivity, and reproducibility of multiplex protein microarray immunoassays, in addition to reducing background fluorescence and maximizing signal-to-noise ratios.