PolyAn Plex bead kits provide a platform for the design of multiplexed suspension arrays that can be run on standard flow cytometers. PolyAn offers a 25-plex (peaks) set of beads that can be distinguished by both different fluorescence intensities of our PolyAn Red4 dye (Excitation: 590–680 nm/Emission: 660–780 nm) as well as three different sizes (3.5 μm, 5 μm and 9 μm).
The standard packaging volume is 1.5 mL/population with a solids content of 0.5%. A custom modification with antibodies, peptides or oligonucleotides is available upon request.
The image below illustrates the read-out of all 25 populations of the PolyAn Plex Red4 beads at one setting using different flow cytometers.
Images courtesy of Helmholtz Zentrum München (Monoclonal Antibody Core Facility & Research Group) and **Humboldt Universität Berlin (Systems Immunology Lab), respectively.
- Egia-Mendikute, L., Bosch, A., Prieto-Fernández, E. et al. Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals, 2021, Commun Biol 4, 485.