PolyAn Red4 Multiplex Beads

PolyAn Red4 Multiplex Beads provide a platform for the design of multiplexed assays that can be run on a wide range of standard flow cytometers.

PolyAn offers a set of 25 bead populations (25-plex) that can be distinguished both by different fluorescence intensities of our PolyAn Red4 dye in the APC channel (Excitation 590–680 nm, Emission 660–780 nm) and three different bead sizes: 3.5 μm, 5.5 μm, and 8.5 μm.

The image below illustrates the read-out of all 25 populations of the PolyAn Red4 Multiplex Beads using different flow cytometers (left: Beckman Coulter CytoFlex-LX, right: Quantum Analysis Quantum-P).

Principle of Bead-based Immunoassays

The principle of bead-based sandwich immunoassays for the detections of antigens or antibodies using PolyAn Red4 Multiplex Beads and flow cytometry is illustrated below.

Each Red4 bead population is encoded with a specific fluorescence intensity, enabeling the classification of the bead populations by their different emission intensities. As each Red4 bead population is labeled with specific capture molecules (e.g. antibodies or antigens), the respective analytes specifically bind to their corresponding "Capture-Beads" and can then be quantified via the emission intensity of a reporter dye.

A flyer showing the use of PolyAn Red4 Multiplex Beads for the multiplexed detection in immunoassays using flow cytometry can be downloaded here.

Products

Id
Title
106 50 003 3.5µm, 3D-Carboxy PolyAn Red4 Multiplex Beads, 8 populations (peaks)
106 50 005 5µm, 3D-Carboxy PolyAn Red4 Multiplex Beads, 10 populations (peaks)
106 50 009 8.5µm, 3D-Carboxy PolyAn Red4 Multiplex Beads, 7 populations (peaks)
106 51 003 3.5µm, 3D-Alkyne PolyAn Red4 Multiplex Beads, 8 populations (peaks)
106 51 005 5µm, 3D-Alkyne PolyAn Red4 Multiplex Beads, 10 populations (peaks)
106 51 009 8.5µm, 3D-Alkyne PolyAn Red4 Multiplex Beads, 7 populations (peaks)
106 52 003 3.5µm, Streptavidin PolyAn Red4 Multiplex Beads, 8 populations (peaks)
106 52 005 5µm, Streptavidin PolyAn Red4 Multiplex Beads, 10 populations (peaks)
106 52 009 8.5µm, Streptavidin PolyAn Red4 Multiplex Beads, 7 populations (peaks)
106 53 003 3.5µm, Neutravidin PolyAn Red4 Multiplex Beads, 8 populations (peaks)
106 53 005 5µm, Neutravidin PolyAn Red4 Multiplex Beads, 10 populations (peaks)
106 53 009 8.5µm, Neutravidin PolyAn Red4 Multiplex Beads, 7 populations (peaks)
106 54 003 3.5µm, 3D-Aldehyde PolyAn Red4 Multiplex Beads, 8 populations (peaks)
106 54 005 5µm, 3D-Aldehyde PolyAn Red4 Multiplex Beads, 10 populations (peaks)
106 54 009 8.5µm, 3D-Aldehyde PolyAn Red4 Multiplex Beads, 7 populations (peaks)
106 55 003 3.5µm, 3D-Azide PolyAn Red4 Multiplex Beads, 8 populations (peaks)
106 55 005 5µm, 3D-Azide PolyAn Red4 Multiplex Beads, 10 populations (peaks)
106 55 009 8.5µm, 3D-Azide PolyAn Red4 Multiplex Beads, 7 populations (peaks)
106 56 003 3.5µm, Protein A/G PolyAn Red4 Multiplex Beads, 8 populations (peaks)
106 56 005 5µm, Protein A/G PolyAn Red4 Multiplex Beads, 10 populations (peaks)
106 56 009 8.5µm, Protein A/G PolyAn Red4 Multiplex Beads, 7 populations (peaks)
106 57 003 3.5µm, 3D-DBCO PolyAn Red4 Multiplex Beads, 8 populations (peaks)
106 57 005 5µm, 3D-DBCO PolyAn Red4 Multiplex Beads, 10 populations (peaks)
106 57 009 8.5µm, 3D-DBCO PolyAn Red4 Multiplex Beads, 7 populations (peaks)
106 58 003 3.5µm, 3D-MTZ PolyAn Red4 Multiplex Beads, 8 populations (peaks)
106 58 005 5µm, 3D-MTZ PolyAn Red4 Multiplex Beads, 10 populations (peaks)
106 58 009 8.5µm, 3D-MTZ PolyAn Red4 Multiplex Beads, 7 populations (peaks)
Request a quote for the selected products

The standard packaging volume is 1.5 mL/population with a solids content of 0.5%. A custom modification with antibodies, peptides or oligonucleotides is available upon request.

Selected Publications and References

  • Kutzner, K. et al., `Phosphorylation of serine-893 in CARD11 suppresses the formation and activity of the CARD11-BCL10-MALT1 complex in T and B cells´, Sci. Signal. 2022, 15, 3083. DOI: 10.1126/scisignal.abk3083.

  • Egia-Mendikute, L. et al., `Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals´, Commun. Biol. 2021, 4, 485. DOI: 10.1038/s42003-021-02011-6.