PolyAn Red4 Multiplex Beads provide a platform for the design of multiplexed assays that can be run on a wide range of standard flow cytometers.
PolyAn offers a set of 25 bead populations (25-plex) that can be distinguished both by different fluorescence intensities of our PolyAn Red4 dye in the APC channel (Excitation 590–680 nm, Emission 660–780 nm) and three different bead sizes: 3.5 μm, 5.5 μm, and 8.5 μm.
The image below illustrates the read-out of all 25 populations of the PolyAn Red4 Multiplex Beads using different flow cytometers (left: Beckman Coulter CytoFlex-LX, right: Quantum Analysis Quantum-P).
The principle of bead-based sandwich immunoassays for the detections of antigens or antibodies using PolyAn Red4 Multiplex Beads and flow cytometry is illustrated below.
Each Red4 bead population is encoded with a specific fluorescence intensity, enabeling the classification of the bead populations by their different emission intensities. As each Red4 bead population is labeled with specific capture molecules (e.g. antibodies or antigens), the respective analytes specifically bind to their corresponding "Capture-Beads" and can then be quantified via the emission intensity of a reporter dye.
A flyer showing the use of PolyAn Red4 Multiplex Beads for the multiplexed detection in immunoassays using flow cytometry can be downloaded here.
The standard packaging volume is 1.5 mL/population with a solids content of 0.5%. A custom modification with antibodies, peptides or oligonucleotides is available upon request.
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Egia-Mendikute, L. et al., `Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals´, Commun. Biol. 2021, 4, 485. DOI: 10.1038/s42003-021-02011-6.