PolyAn Red4 encoded Multiplex Beads

PolyAn Plex bead kits provide a platform for the design of multiplexed suspension arrays that can be run on standard flow cytometers. PolyAn offers a 25-plex (peaks) set of beads that can be distinguished by both different fluorescence intensities of our PolyAn Red4 dye (Excitation: 590–680 nm/Emission: 660–780 nm) as well as three different sizes (3.5 μm, 5 μm and 9 μm).

Products

IdMean DiameterSurface ModificationsColor Labeling
106 50 0033.5 µm3D-Carboxy AFPolyAn Red4
106 50 0055 µm3D-Carboxy AFPolyAn Red4
106 50 0098.5 µm3D-Carboxy AFPolyAn Red4
106 51 0033.5 µm3D-AlkynePolyAn Red4
106 51 0055 µm3D-AlkynePolyAn Red4
106 51 0098.5 µm3D-AlkynePolyAn Red4
106 52 0033.5 µmStreptavidinPolyAn Red4
106 52 0055 µmStreptavidinPolyAn Red4
106 52 0098.5 µmStreptavidinPolyAn Red4
106 53 0033.5 µmNeutravidinPolyAn Red4
106 53 0055 µmNeutravidinPolyAn Red4
106 53 0098.5 µmNeutravidinPolyAn Red4
106 54 0033.5 µm3D-AldehydePolyAn Red4
106 54 0055 µm3D-AldehydePolyAn Red4
106 54 0098.5 µm3D-AldehydePolyAn Red4
106 55 0033.5 µm3D-AzidePolyAn Red4
106 55 0055 µm3D-AzidePolyAn Red4
106 55 0098.5 µm3D-AzidePolyAn Red4
106 56 0033.5 µmProtein A/GPolyAn Red4
106 56 0055 µmProtein A/GPolyAn Red4
106 56 0098.5 µmProtein A/GPolyAn Red4
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The standard packaging volume is 1.5 mL/population with a solids content of 0.5%. Our PolyAn Plex beads are also available with Protein A/G and 3D-Azide surfaces. A custom modification with antibodies, peptides or oligonucleotides is available upon request.

The image below illustrates the read-out of all 25 populations of the PolyAn Plex Red4 beads at one setting using different flow cytometers.

Images courtesy of *Systems Immunology Lab (Humboldt Universität Berlin) and **Quantum Analysis GmbH, respectively.