PolyAn Red4 Multiplex Beads
PolyAn Multiplex Beads provide a platform for the design of multiplexed assays that can be run on standard flow cytometers.
PolyAn offers a set of 25 bead populations (25-plex) that can be distinguished both by different fluorescence intensities of our PolyAn Red4 dye in the APC channel (Excitation 590–680 nm, Emission 660–780 nm) and three different bead sizes: 3.5 μm, 5.5 μm, and 8.5 μm.
The image below illustrates the read-out of all 25 populations of the PolyAn Red4 Multiplex Beads using different flow cytometers (left: Beckman Coulter CytoFlex-LX, right: Quantum Analysis Quantum-P).
The standard packaging volume is 1.5 mL/population with a solids content of 0.5%. A custom modification with antibodies, peptides or oligonucleotides is available upon request.
Selected Publications and References
- Kutzner, K. et al., `Phosphorylation of serine-893 in CARD11 suppresses the formation and activity of the CARD11-BCL10-MALT1 complex in T and B cells´, Sci. Signal., 2022, 15, 3083. DOI: 10.1126/scisignal.abk3083.
Egia-Mendikute, L. et al., `Sensitive detection of SARS-CoV-2 seroconversion by flow cytometry reveals the presence of nucleoprotein-reactive antibodies in unexposed individuals´, Commun. Biol., 2021, 4, 485. DOI: 10.1038/s42003-021-02011-6.