Protein Microarrays

Protein microarrays come in two basic formats, forward and reverse phase. In "forward phase" arrays, captured reagents - whether antibodies, aptamers, or other proteins  - are arrayed at defined positions on a glass slide or similar substrate, which is then interrogated with any of a variety of probes, from protein lysate and enzymes to small molecules and nucleic acids.

On the other hand, with a reverse phase array protein lysates are spotted. The arrays are then probed with antibodies, for instance, phosphorylated signaling molecules - one antibody probing multiple samples as opposed to one sample for multiple capture reagents.

Transparent, reactive glass slides for Protein Microarrays

All of PolyAn’s reactive surfaces are completely transparent. They are characterized by a low lot-to-lot variation that is specified and monitored by using contact angle measurements as well as qualitative test methods.

Id
Title
Surface Modifications
Id Title Surface Modifications
104 00 927 Surface test package (6) with 3x5 polymer slides for immobilization of proteins 3D-Epoxy, 3D-Aldehyde and 3D-NHS
104 00 950 Surface test package (1) with 3x5 glass slides for immobilization of proteins 2D-Epoxy, 2D-Aldehyde and 3D-NHS
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More hydrophobic surfaces may result in reduced spot diameter, depending on the spotting buffer composition. Results may vary based on buffers, sample preparation, spotting and scanning instruments.

Please note, that in order to facilitate handling of the glass slides PolyAn also offers a range of useful accessories and reagents.

Nitrocellulose Film Slides

PolyAn is the distributor of the nitrocellulose film slides from Grace Bio-Labs. The following table illustrates the different product families for RPPA and other microarray applications:

 

 

AVID

NOVA

SuperNOVA

PATH

Binding Capacity
 
++++ ++ ++++ +
Fluorescence background + ++ +++ ++++
Dynamic range (log scale fluorescence) 5-6 5-6 7+ 4-5
Hydrophobicity
 
+ + + ++
Applications Best for any application requiring high binding capacity and colorimetric detection. Reduced fluorescence background with lower binding capacity than AVID. Good signal-to-noise ratio for fluorescence detection. Second generation NOVA, lowest fluorescence background, high binding capacity. Best for fluorescence detection and large dynamic range. Lowest fluorescence background, lower binding capacity, reduced dynamic range. Best signal-to-noise ratio for fluorescence detection.