Protein Microarrays

Protein microarrays come in two basic formats, forward and reverse phase. In "forward phase" arrays, captured reagents - whether antibodies, aptamers, or other proteins  - are arrayed at defined positions on a glass slide or similar substrate, which is then interrogated with any of a variety of probes, from protein lysate and enzymes to small molecules and nucleic acids.

On the other hand, with a reverse phase array protein lysates are spotted. The arrays are then probed with antibodies, for instance, phosphorylated signaling molecules - one antibody probing multiple samples as opposed to one sample for multiple capture reagents.

All of PolyAn’s reactive surfaces are completely transparent. They are characterized by a low lot-to-lot variation that is specified and monitored by using contact angle measurements as well as qualitative test methods.

More hydrophobic surfaces may result in reduced spot diameter, depending on the spotting buffer composition. Results may vary based on buffers, sample preparation, spotting and scanning instruments.

Please note, that in order to facilitate handling of the glass slides PolyAn also offers a range of useful accessories and reagents.

Selected Publications

• Allelein, S. et al., `Prostate-Specific Membrane Antigen (PSMA)-Positive Extracellular Vesicles in Urine: A Potential Liquid Biopsy Strategy for Prostate Cancer Diagnosis?´, Cancers 2022, 14, 2987. DOI: 10.3390/cancers14122987.
• Itri, S. et al., `A pin-based pyro-electrohydrodynamic jet sensor for tuning the accumulation of biomolecules down to sub-picogram level detection´, Sens. Biosens. Res. 2022, 38, 100536. DOI: 10.1016/j.sbsr.2022.100536.
• Horta, S. et al., `Evaluation of Immuno-Rolling Circle Amplification for Multiplex Detection and Profiling of Antigen-Specific Antibody Isotypes´, Anal. Chem. 2021, 93, 6169. DOI: 10.1021/acs.analchem.1c00172.
• Malik, A. et al., `Immunological Evaluation of Synthetic Glycosylphosphatidylinositol Glycoconjugates as Vaccine Candidates against Malaria´, Chem. Biol. 2020, 15, 171. DOI: 10.1021/acschembio.9b00739.
• Goyette, A.-P. et al., `Microfluidic multipoles theory and applications´, Nature Commun. 2019, 10, 1781. DOI: 10.1038/s41467-019-09740-7.
• Hettegger, P. et al., `High similarity of IgG antibody profiles in blood and saliva opens opportunities for saliva based serology´, PLoS One 2019, 14, 218456. DOI: 10.1371/journal.pone.0218456.
• Herrmann; A. et al., `Bioorthogonal in Situ Hydrogels Based on Polyether Polyols for New Biosensor Materials with High Sensitivity´, Appl. Mater. Interfaces 2018, 10, 11382. DOI: 10.1021/acsami.8b01860.
• Peter, H. et al., `Lab-on-a-Chip Device for Rapid Measurement of Vitamin D Levels´, Meth. Mol. Biol. 2018, 35, 477. DOI: 10.1007/978-1-4939-7614-0_35.
• Moscetti, I. et al., `Binding kinetics of mutant p53R175H with wild type p53 and p63: A Surface Plasmon Resonance and Atomic Force Spectroscopy study´, Biophys. Chem. 2017, 228, 55. DOI: 10.1016/j.bpc.2017.07.002.
• Peter, H. et al., `Lab-on-a-Chip Proteomic Assays for Psychiatric Disorders´, Adv. Exp. Med. Biol. 2017, 33, 339. DOI: 10.1007/978-3-319-52479-5_33.
• Soria, J. et al., `Tear proteome analysis in ocular surface diseases using label-free LC-MS/MS and multiplexed microarray biomarker validation´, Sci. Reports 2017, 7, 17478. DOI: 10.1038/s41598-017-17536-2.
• Gerdtsson, A.S. et al., `Evaluation of Solid Supports for Slide- and Well-Based Recombinant Antibody Microarrays´, Microarrays 2016, 5, 16. DOI: 10.3390/microarrays5020016.