for coupling via electrostatical adsorption of negatively charged biochemical species
An adsorptive immobilization is a non-covalent coupling method on solid supports which is realized by electrostatic, Van der Waals interactions, hydrogen bonds and hydrophobic interactions of the reactants, respectively. An electrostatic interaction is formed by an ion-ion-interaction between the surface and the applied biochemical species. The dissociation energy for typical electrostatic bond is 130 kJ/mol. It is about a third of the strength of an average covalent bond. In order to achieve an optimal adhesion the probe buffer und the adhesion conditions (pH-value) have to be optimized.
Binding of oligonucleotides on the 3D-Amino matrix
The nucleic acid is bound electrostatically on the 3D-Amino surface with its negatively charged backbone or its 5´phosphate group. For immobilization of nulceic acids we recommend an UV- crosslinking after adsorption forming a covalent bond. During the UV irradiation the base Thymine formes radicals which undergoes a H-abstraction in the 3D- Matrix.
PolyAn equips glass slides and polymer slides with 3D-Amino surfaces. Additionally, PolyAn has a range of porous materials (membranes, fleece and sinter materials) and microparticles in our product portfolio that are functionalised with 3D-Amino surfaces.
Please do not hesitate to contact us, if you would like to functionalise a different format or substrate with our 3D-Amino surface.