for coupling via electrostatical adsorption of negatively charged biochemical species
An adsorptive immobilization is a non-covalent coupling method on solid supports which is realized by electrostatic and Van-der-Waals interactions as well as hydrogen bonds and hydrophobic interactions of the reactants. An electrostatic interaction is formed by an ion-ion-interaction between the surface and the applied biochemical species. The dissociation energy for typical electrostatic bond is 130 kJ/mol, which is about a third of the strength of an average covalent bond. In order to achieve an optimal adhesion the probe buffer und the adhesion conditions (pH-value) have to be optimized.
Binding of oligonucleotides on the 3D-Amino matrix
Oligonucleotides can be bound electrostatically on the 3D-Amino surface with their negatively charged backbone or their 5´-phosphate group. For immobilization of oligonucleotides we recommend an additional UV-crosslinking after adsorption. During the UV irradiation the nucleic base Thymine forms radicals which undergo H-abstraction in the 3D-Matrix to form a covalent bond.
PolyAn equips glass slides, coverslips and polymer slides with 3D-Amino surfaces. Additionally, PolyAn has a range of porous materials (membranes, fleece and sinter materials) and microparticles in our product portfolio that are functionalised with 3D-Amino surfaces.
Please do not hesitate to contact us, if you would like to functionalise a different format or substrate with our 3D-Amino surface.