3D-Azide surface chemistry

for bio-orthogonal binding of oligonucleotides, peptides, and small molecules containing alkyne or cyclo-alkyne groups via Click chemistry.

Key Features

  • Simple coupling chemistry, highly specific bio-orthogonal reaction
  • Alternative to conventional Streptavidin-Biotin immobilization
  • Three-dimensional (3D)-surface chemistry comprised of a long-chain polymer containing a defined number of azide groups. The 3D-structure incorporates moieties that reduce unspecific binding
  • Polypropylene plates available for applications using organic solvents or aggressive media

PolyAn equips conventional 96-well plates as well as solvent stable Polypropylene plates with 3D-Azide surfaces. Please do not hesitate to contact us, if you would like to functionalize a different format or substrate with our 3D-Azide surface.

3D-Azide surface for DNA hybridization

Measurements to demonstrate and verify the efficient immobilization of DBCO-modified oligonucleotides to 3D-Azide microplates via copper-free click-chemistry.

Left: Immobilization of a 5’-DBCO-oligonucleotide (20mer, 3'-labelled with 6-FAM) via strain promoted alkyne-azide cycloaddition (SPAAC), Right: Hybridization with the corresponding anti-strand (5’-labelled with TAMRA).