Microarray Slides for Peptide applications

Peptide Microarrays are two-dimensional arrays of peptides printed onto a solid substrate used to assay a large number of analytes within a sample for multiplexed high-throughput screening.

All of PolyAn’s reactive surfaces are completely transparent. They are characterized by a low lot-to-lot variation that is specified and monitored by using contact angle measurements as well as qualitative test methods.

A flyer showing the most commonly used functionalized surfaces for Peptide Microarrays available from PolyAn can be downloaded here.

Products

Id
Title
Surface Modifications
104 00 915 Surface test package (2) with 3x5 glass slides for directed immobilisation of peptides Streptavidin, Neutravidin and 3D-Maleimid
104 00 930 Surface test package (1) with 3x5 glass slides for immobilization of peptides 2D-Epoxy, 3D-Epoxy and 3D-Amino
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More hydrophobic surfaces may result in reduced spot diameter, depending on the spotting buffer composition. Results may vary based on buffers, sample preparation, spotting and scanning instruments.

Please note, that in order to facilitate handling of the glass slides PolyAn also offers a range of useful accessories and reagents.

Selected Publications

  • Cai, F. et al., `Structural and genetic convergence of HIV-1 neutralizing antibodies in vaccinated nonhuman primates´, PLoS Pathogens 2021, 17, 1009624. DOI: 10.1371/journal.ppat.1009624.
  • Saunders, K.O. et al., `Lipid nanoparticle encapsulated nucleoside-modified mRNA vaccines elicit polyfunctional HIV-1 antibodies comparable to proteins in nonhuman primates´, npj Vaccines 2021, 6, 50. DOI: 10.1038/s41541-021-00307-6.
  • Gorini, G. et al., `Engagement of monocytes, NK cells, and CD4+ Th1 cells by ALVAC-SIV vaccination results in a decreased risk of SIVmac251 vaginal acquisition´, PLoS Pathogens 2020, 16, 1008377. DOI: 10.1371/journal.ppat.1008377.
  • Schneider, J.R. et al., `A MUC16 IgG Binding Activity Selects for a Restricted Subset of IgG Enriched for Certain Simian Immunodeficiency Virus Epitope Specificities´, J. Virol. 2020, 94, 01246-19. DOI: 10.1128/JVI.01246-19.
  • Shen, X. et al., `HIV-1 Vaccine Sequences Impact V1V2 Antibody Responses: A Comparison of Two Poxvirus Prime gp120 Boost Vaccine Regimens´, Sci. Reports 2020, 10, 2093. DOI: 10.1038/s41598-020-57491-z.
  • Dennis, M. et al., `Coadministration of CH31 Broadly Neutralizing Antibody Does Not Affect Development of Vaccine-Induced Anti-HIV-1 Envelope Antibody Responses in Infant Rhesus Macaques´, J. Virol. 2019, 93, 01783-18. DOI: 10.1128/JVI.01783-18.
  • Han, Q. et al., `Difficult-to-neutralize global HIV-1 isolates are neutralized by antibodies targeting open envelope conformations´, Nature Commun. 2019, 10, 2898. DOI: 10.1038/s41467-019-10899-2.
  • Jones, A.T. et al., `HIV-1 vaccination by needle-free oral injection induces strong mucosal immunity and protects against SHIV challenge´, Nature Commun. 2019, 10, 798. DOI: 10.1038/s41467-019-08739-4.
  • Nelson, A.N. et al., `Simian-Human Immunodeficiency Virus SHIV.CH505-Infected Infant and Adult Rhesus Macaques Exhibit Similar Env-Specific Antibody Kinetics, despite Distinct T-Follicular Helper and Germinal Center B Cell Landscapes´, J. Virol. 2019, 93, 00168-19. DOI: 10.1128/JVI.00168-19.
  • Randriantsilefisoa, R. et al., `Highly sensitive detection of antibodies in a soft bioactive three-dimensional bioorthogonal hydrogel´, J. Mater. Chem. B 2019, 7, 3220. DOI: 10.1039/c9tb00234k.
  • Hsu, C.-C. et al., `Gas41 links histone acetylation to H2A.Z deposition and maintenance of embryonic stem cell identity´, Cell Discovery 2018, 4, 28. DOI: 10.1038/s41421-018-0027-0.
  • Schiffner, T. et al., `Structural and immunologic correlates of chemically stabilized HIV-1 envelope glycoproteins´, PLoS Pathogens 2018, 14, 1006986. DOI: 10.1371/journal.ppat.1006986.
  • Wen, Y. et al., `Generation and characterization of a bivalent protein boost for future clinical trials: HIV-1 subtypes CR01_AE and B gp120 antigens with a potent adjuvant´, PLOS one 2018, 13, 194266. DOI: 10.1371/journal.pone.0194266.
  • Phillips, B. et al., `Impact of Poxvirus Vector Priming, Protein Coadministration, and Vaccine Intervals on HIV gp120 Vaccine-Elicited Antibody Magnitude and Function in Infant Macaques´, Clin. Vacc. Immun. 2017, 24, 00231-17. DOI: 10.1128/CVI.00231-17.
  • Shen, X. et al., `HIV-1 gp120 and Modified Vaccinia Virus Ankara (MVA) gp140 Boost Immunogens Increase Immunogenicity of a DNA/MVA HIV-1 Vaccine´, J. Virol. 2017, 91, 01077-17. DOI: 10.1128/JVI.01077-17.
  • Shen, X. et al., `Cross-Linking of a CD4-Mimetic Miniprotein with HIV-1 Env gp140 Alters Kinetics and Specificities of Antibody Responses against HIV-1 Env in Macaques´, J. Virol. 2017, 91, 00401-17. DOI: 10.1128/JVI.00401-17.
  • Zurawski, G. et al., `Superiority in Rhesus Macaques of Targeting HIV-1 Env gp140 to CD40 versus LOX-1 in Combination with Replication-Competent NYVAC-KC for Induction of Env-Specific Antibody and T Cell Responses´, J. Virol. 2017, 91, 01596-16. DOI: 10.1128/JVI.01596-16.
  • Striffler, J. et al., `Replication of Polymer-Based Peptide Microarrays by Multi-Step Transfer´, Chem. Nano. Mat. 2016, 2, 897. DOI: 10.1002/cnma.201600194.
  • Rohe, A. et al., `Identification of peptidic substrates for the human kinase Myt1 using peptide microarrays´, Bioorg. Med. Chem. 2015, 23, 4936. DOI: 10.1016/j.bmc.2015.05.021.
  • Stephenson, K.E. et al., `Quantification of the epitope diversity of HIV-1-specific binding antibodies by peptide microarrays for global HIV-1 vaccine development´, J. Immunol. Meth. 2015, 416, 105. DOI: 10.1016/j.jim.2014.11.006.
  • Schönberg, A. et al., `The Peptide Microarray ‘‘ChloroPhos1.0’’ Identifies New Phosphorylation Targets of Plastid Casein Kinase II (pCKII) in Arabidopsis thaliana´, PLoS One 2014, 9, 108344. DOI: 10.1371/journal.pone.0108344.
  • Rauh, D. et al., `An acetylome peptide microarray reveals specificities and deacetylation substrates for all human sirtuin isoforms´, Nature Commun. 2013, 4, 2327. DOI: 10.1038/ncomms3327.