Dual color encoded Multiplex Beads

The spectral characteristics of the dyes used for the dual colour encoded system are:

Color 1:Excitation 420 - 480 nm   Color 2:Excitation 515 - 540 nm
Emission 485 - 540 nmEmission 535 - 570 nm


PolyAn's dual color encoded have been developed specifically for read-out using a fluorescence microscope in combination with a suitable pattern recognition software.

Products

IdMean DiameterColor LabelingSurface Modifications
105 12 01111 µmdual colour3D-Carboxy AF
105 12 01515 µmdual colour3D-Carboxy AF
105 31 01111 µmdual colourStreptavidin
105 31 01515 µmdual colourStreptavidin
105 32 01111 µmdual colourNeutravidin
105 32 01515 µmdual colourNeutravidin
105 33 01111 µmdual colour3D-Alkyne
105 33 01515 µmdual colour3D-Alkyne
105 34 01111 µmdual colour3D-Azide
105 34 01515 µmdual colour3D-Azide
105 35 01111 µmdual colourProtein A/G
105 35 01515 µmdual colourProtein A/G
105 36 01111 µmdual colour3D-Aldehyde
105 36 01515 µmdual colour3D-Aldehyde
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The standard packaging volume is 1.5 mL/population with a solids content of 0.5%. Please do not hesitate to contact us, if you are interested in a set of multiplex bead populations that is tailored to your specific application and/or read-out system.

Technology for dual-color bead encoding

The definition and differentiation between bead populations used in multiplex bead assays can be achieved using a combination of fluorescence emission wavelength (colour), fluorescence intensity and bead size. This encoding principle is illustrated in the image above.

PolyAn offers both dual-colour encoding and single-dye-encoding. Dual-colour encoded beads allow a narrower definition of the bead population, but have higher requirements regarding the read-out software.

By combining fluorescence encoding and different particle size populations up to 100 populations can be defined. Using size as well as fluorescence makes it easier to differentiate between populations. The overall system becomes more robust and the requirements with regards to the read-out system can be reduced.

Selected publications

  • C. Liebsch, S. Roediger, A. Boehm, J. Nitschke, J. Weinreich, A. Fruth, D. Roggenbuck, W. Lehmann, U. Schedler, T. Juretzek, P. Schierack, Solid-phase microbead array for the multiplex O-serotyping of Escherichia coli, Microchim Acta 2017 184:1405–1415.
  • Scholz, J., Grossmann, K., Knütter, I., Hiemann, R., Sowa, M., Röber, N., Rödiger, S., Schierack, P., Reinhold, D., Bogdanos, D., Meroni, P.L., Radice, A., Conrad, K., Roggenbuck, D. (2015). Second generation analysis of antinuclear antibody (ANA) by combination of screening and confirmatory testing. Clin Chem Lab Med. May 15.
  • Spiess, A.N., Deutschmann, C., Burdukiewicz, M., Himmelreich, R., Klat, K., Schierack, P., Rodiger, S. (2015). Impact of Smoothing on Parameter Estimation in Quantitative DNA Amplification Experiments. Clinical Chemistry. Feb;61(2):379-88.
  • Sowa, M., Großmann, K., Scholz, J., Röber, N., Rödiger, S., Schierack, P., Conrad, K., Roggenbuck, D., Hiemann, R. (2014). Der CytoBead-Assay – Eine neue Möglichkeit der multiparametrischen Autoantikörperanalytik bei systemischen Autoimmunerkrankungen. J Lab Med 2014; 38(6): 309–317.
  • Rödiger, S., Liebsch, C., Schmidt, C, Lehman, W., Resch-Genger, U., Schedler, U., Schierack, P. (2014). Nucleic acid detection based on the use of microbeads: a review. Microchimica Acta. Volume 181, Issue 11-12, pp 1151-1168.
  • Rödiger, S., Lehmann, W., Schierack, P., Schröder, C. (2013) Mikropartikelsysteme für die Nukleinsäurediagnostik. BioSpectrum, 02/2013, 153-154
  • Schierack, P., Rödiger, S., Kuhl, C., Hiemann, R., Roggenbuck, D., Li, G., Weinreich, J., Berger, E., Nolan, L.K., Nicholson, B., Römer, A., Frömmel, U., Wieler, L.H., Schröder, C. (2013). Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method. PLoS One. 2013 Apr 26;8(4):e59242.
  • Rödiger, S., Schierack, P., Böhm, A., Nitschke, J., Berger, I., Frömmel, U., Schmidt, C., Ruhland, M., Schimke, I., Roggenbuck, D., Lehmann, W., Schröder, C. (2013). A highly versatile microscope imaging technology platform for the multiplex real-time detection of biomolecules and autoimmune antibodies. Adv Biochem Eng Biotechnol. 133:35-74.
  • Grossmann, K., Roggenbuck, D., Schröder, C., Conrad, K., Schierack, P., Sack, U. (2011). Multiplex assessment of non-organ-specific autoantibodies with a novel microbead-based immunoassay. Cytometry A. Feb;79(2):118-25.
  • Frömmel, U., Berger, I., Rödiger, S., Schierack, P., Schröder, C., Multiplex-PCR Mikropartikel-Assay zum Nachweis bakterieller Gene, 01/2011; Pabst Publishers, ISBN: 978-3-89967-703-4