Dual-color encoded Multiplex Beads

The definition and differentiation between bead populations used in multiplex bead assays can be achieved using a combination of fluorescence emission wavelength (color), fluorescence intensity, and bead size. By combining fluorescence encoding and different particle size populations up to 100 populations can be defined.

PolyAn's dual-color encoded beads have been developed specifically for read-out using a fluorescence microscope in combination with a suitable pattern recognition software. This encoding principle is illustrated in the image below. 

Dual-color encoded beads allow a narrower definition of the bead population, but have higher requirements regarding the read-out software. Using size as well as fluorescence makes it easier to differentiate between populations. The overall system becomes more robust and the requirements with regards to the read-out system can be reduced.

The standard packaging volume is 1.5 mL/population with a solids content of 0.5%.

PolyAn offers both dual-color encoding and single-dye-encoding. The spectral characteristics of the dyes used for the dual-color encoded system are:

Please do not hesitate to contact us, if you are interested in a set of multiplex bead populations that is tailored to your specific application and/or read-out system.

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