Dual-color encoded Multiplex Beads

The definition and differentiation between bead populations used in multiplex bead assays can be achieved using a combination of fluorescence emission wavelength (color), fluorescence intensity, and bead size. By combining fluorescence encoding and different particle size populations up to 100 populations can be defined.

PolyAn's dual-color encoded beads have been developed specifically for read-out using a fluorescence microscope in combination with a suitable pattern recognition software. This encoding principle is illustrated in the image below. 


Dual-color encoded beads allow a narrower definition of the bead population, but have higher requirements regarding the read-out software. Using size as well as fluorescence makes it easier to differentiate between populations. The overall system becomes more robust and the requirements with regards to the read-out system can be reduced.


Mean Diameter
Color Labeling
Surface Modifications
Id Mean Diameter Color Labeling Surface Modifications
105 12 011 11 µm dual colour 3D-Carboxy AF
105 12 015 15 µm dual colour 3D-Carboxy AF
105 31 011 11 µm dual colour Streptavidin
105 31 015 15 µm dual colour Streptavidin
105 32 011 11 µm dual colour Neutravidin
105 32 015 15 µm dual colour Neutravidin
105 33 011 11 µm dual colour 3D-Alkyne
105 33 015 15 µm dual colour 3D-Alkyne
105 34 011 11 µm dual colour 3D-Azide
105 34 015 15 µm dual colour 3D-Azide
105 35 011 11 µm dual colour Protein A/G
105 35 015 15 µm dual colour Protein A/G
105 36 011 11 µm dual colour 3D-Aldehyde
105 36 015 15 µm dual colour 3D-Aldehyde
Request a quote for the selected products

The standard packaging volume is 1.5 mL/population with a solids content of 0.5%.

PolyAn offers both dual-color encoding and single-dye-encoding. The spectral characteristics of the dyes used for the dual-color encoded system are:

Color 1: Excitation 420 - 480 nm    Color 2: Excitation 515 - 540 nm
  Emission 485 - 540 nm   Emission 535 - 570 nm

Please do not hesitate to contact us, if you are interested in a set of multiplex bead populations that is tailored to your specific application and/or read-out system.

Selected publications

  • C. Liebsch, S. Roediger, A. Boehm, J. Nitschke, J. Weinreich, A. Fruth, D. Roggenbuck, W. Lehmann, U. Schedler, T. Juretzek, P. Schierack, Solid-phase microbead array for the multiplex O-serotyping of Escherichia coli, Microchim Acta 2017 184:1405–1415.
  • Scholz, J., Grossmann, K., Knütter, I., Hiemann, R., Sowa, M., Röber, N., Rödiger, S., Schierack, P., Reinhold, D., Bogdanos, D., Meroni, P.L., Radice, A., Conrad, K., Roggenbuck, D. (2015). Second generation analysis of antinuclear antibody (ANA) by combination of screening and confirmatory testing. Clin Chem Lab Med. May 15.
  • Spiess, A.N., Deutschmann, C., Burdukiewicz, M., Himmelreich, R., Klat, K., Schierack, P., Rodiger, S. (2015). Impact of Smoothing on Parameter Estimation in Quantitative DNA Amplification Experiments. Clinical Chemistry. Feb;61(2):379-88.
  • Sowa, M., Großmann, K., Scholz, J., Röber, N., Rödiger, S., Schierack, P., Conrad, K., Roggenbuck, D., Hiemann, R. (2014). Der CytoBead-Assay – Eine neue Möglichkeit der multiparametrischen Autoantikörperanalytik bei systemischen Autoimmunerkrankungen. J Lab Med 2014; 38(6): 309–317.
  • Rödiger, S., Liebsch, C., Schmidt, C, Lehman, W., Resch-Genger, U., Schedler, U., Schierack, P. (2014). Nucleic acid detection based on the use of microbeads: a review. Microchimica Acta. Volume 181, Issue 11-12, pp 1151-1168.
  • Rödiger, S., Lehmann, W., Schierack, P., Schröder, C. (2013) Mikropartikelsysteme für die Nukleinsäurediagnostik. BioSpectrum, 02/2013, 153-154
  • Schierack, P., Rödiger, S., Kuhl, C., Hiemann, R., Roggenbuck, D., Li, G., Weinreich, J., Berger, E., Nolan, L.K., Nicholson, B., Römer, A., Frömmel, U., Wieler, L.H., Schröder, C. (2013). Porcine E. coli: Virulence-Associated Genes, Resistance Genes and Adhesion and Probiotic Activity Tested by a New Screening Method. PLoS One. 2013 Apr 26;8(4):e59242.
  • Rödiger, S., Schierack, P., Böhm, A., Nitschke, J., Berger, I., Frömmel, U., Schmidt, C., Ruhland, M., Schimke, I., Roggenbuck, D., Lehmann, W., Schröder, C. (2013). A highly versatile microscope imaging technology platform for the multiplex real-time detection of biomolecules and autoimmune antibodies. Adv Biochem Eng Biotechnol. 133:35-74.
  • Grossmann, K., Roggenbuck, D., Schröder, C., Conrad, K., Schierack, P., Sack, U. (2011). Multiplex assessment of non-organ-specific autoantibodies with a novel microbead-based immunoassay. Cytometry A. Feb;79(2):118-25.
  • Frömmel, U., Berger, I., Rödiger, S., Schierack, P., Schröder, C., Multiplex-PCR Mikropartikel-Assay zum Nachweis bakterieller Gene, 01/2011; Pabst Publishers, ISBN: 978-3-89967-703-4