Streptavidin and Neutravidin surfaces

PolyAn´s streptavidin or neutravidin functionalized microparticles are suitable for coupling biotin-modified biomolecules, e.g. oligonucleotides, peptides, proteins etc..

PolyAn offers to functionalize the surface with Avidin, Streptavidin and Neutravidin, respectively. The table below illustrates the differences between the variants (data of molecules in solution, not immobilized on bead surface):

 

	Avidin	Streptavidin	Neutravidin
Molecular Weight	67 kDa	53 kDa	60 kDa
Biotin-binding Sites	4	4	4
Isoelectric Point (pl)	10	6.8–7.5	6.3
Specificity	Low	High	Highest
Affinity for Biotin (Kd)	10-15 M	10-15 M	10-15 M
Nonspecific Binding	High	Low	Lowest