Microplates for Click Chemistry
Click chemistry describes quick and irreversible one pot conjugation reactions that have a high reaction specificity, give a high yield of the desired product, and lead to minimal byproducts. Bio-orthogonal reactions are conjugation reactions that do not interfere with biological processes.
PolyAn offers a variety of surfaces that are suitable for bio-orthogonal, oriented conjugation of oligonucleotides, peptides, and other small (bio)molecules via click chemistry:
- 3D-Azide: For reaction with Alkyne groups in a Copper-catalyzed Alkyne-Azide Cycloaddition, or Dibenzocyclooctyne (DBCO) groups in a Strain-promoted Alkyne-Azide Cycloaddition
- 3D-DBCO: For binding of Azide-modified molecules via copper-free click chemistry (Strain-promoted Alkyne-Azide Cycloaddition)
- 3D-MTZ: For the fast and efficient binding of biomolecules that are modified with a trans-Cyclooctene (TCO) group via an Inverse electron- demand Diels Alder reaction
Products
The plates are offered for colorimetric, chemiluminescence and fluorescent detection systems, respectively. Please go to this section for glass bottom plates with a wide range of surfaces and well designs. Additionally, PolyAn offers functionalized, solvent stable microplates that are comprised of Polypropylene (PP) as well as covalently coated Streptavidin and Neutravidin plates.
Customized plate surfaces
Please do not hesitate to contact us, if you require a special surface for binding of your biomolecules that is not listed in the products table. Other plate formats and substrates can be equipped with our surfaces as part of our Molecular Surface Engineering Services. We are also happy to support you in developing suitable protocols for coating on our plates.