Fluorescence detection is a preferred method for many quantitative protein microarray assays in research and diagnostic applications due to its excellent sensitivity and potential for multiplexing. Use of multiplexed fluorescence-based assays on porous nitrocellulose though has been hampered by high backgrounds at shorter excitation wavelengths (such as the 532 nm fluorescence channel).

Grace Bio-Labs has therefore developed ONCYTE^® SuperNOVA microporous nitrocellulose film slides to increase protein microarray assay sensitivity and dynamic range using fluorescent endpoints at multiple wavelengths. Use of SuperNOVA slides can lower the limit of detection with fluorescent endpoints by 3 – 4 orders of magnitude over competing nitrocellulose film slides.

ONCYTE(R) SuperNOVA-Nitrocellulose film slides optimized for low background fluorescence without sacrificing dynamic range. Glass slides are 25 x 75 x 1 mm and have 20 slides in a Pack.