Click chemistry is a chemical paradigm introduced by K. Barry Sharpless in 2001. Click chemistry describes chemical reactions for orthogonal, stereo-specific coupling while working in easily removable or benign solvents. The technology is inspired by the fact that chemical reactions in nature also generate substances by joining small modular units. One prominent example of click-chemistry is the reaction of Alkynes with Azide groups.
PolyAn has developed new 3D-Alkyne and 3D-Azide surfaces for directed and bio-orthogonal coupling of biomolecules:
- Alternative to conventional Streptavidin-Biotin chemistry
- Less unspecific interactions compared to Streptavidin
- No reactions of alkyne with regular biomolecule functionalities, e.g. hydroxy, amino and carboxy
PolyAn can functionalize all the beads from our portfolio with the new 3D-Alkyne and 3D-Azide surfaces, respectively:
- Transparent beads
- Fluorescence encoded beads
- Multiplex bead populations with up to 25 peaks for flow cytometry and fluorescence imaging systems
As part of our functionalization services PolyAn is now also offering the custom modification of beads with oligonucleotides or peptides as well as 3D-MTZ (Methyltetrazine) and 3D-DBCO (Dibenzylcyclooctyne) surfaces, respectively. Please do not hesitate to contact us (firstname.lastname@example.org), if you have any questions regarding our products. We are looking forward to your inquiry.
Transparent PolyAn microparticles with 3D-Alkyne surface coupled to fluorescence labeled azido peptide via ”click chemistry”.
[Fluorescence microscope image: Olympus IX71, 60x: visualisation of positive ligand binding]