Spectrum Calibration Beads for flow cytometers

PolyAn’s Spectrum Calibration Beads are designed for calibration of flow cytometers and other fluorescence imaging systems. Each color encoded PMMA bead population (peak) contains a mixture of fluorophores that allows performance validation at all wavelengths.

8-peak Spectrum Calibration Beads with increasing fluorophore content for all channels. One transparent population (only in FL3 detectable) and 7 fluorescence encoded populations. Measurement with QA Quantum P flow cytometer. Excitation laser line at 488 nm.

Key features

  • Contains a mixture of fluorophores that enable the Spectrum Calibration Beads to be excited at any wavelength from 365 nm to 650 nm.
  • Fluorophores are homogeneously encapsulated in the PMMA matrix. A special shell prevents the leaching of fluorophores.
  • Allows the calibration of the FITC, PE, PE-TR, PE-Cy5, and APC channels (or analogue fluorophores) with the same set of particles.
  • Set of up to 8 similar size microparticle populations (peaks) with different fluorescence intensities.

Products

Id
Title
Solids Content
Id Title Solids Content
107 00 006 6µm, Spectrum Flow Cytometer Calibration Beads, 8 populations (peaks) 0.15%
107 01 006 6 µm, Spectrum Flow Cytometer Calibration Beads, 5 populations (peaks) 0.15%
107 02 006 6 µm, Spectrum Flow Cytometer Calibration Beads, 1 population (peak) 0.15%
107 02 010 10 µm, Spectrum Flow Cytometer Calibration Beads, 1 population (peak) 0.15%
107 03 006 6 µm, Spectrum Flow Cytometer Calibration Beads - SCB 0.2.4, 1 population (peak) 0.15%
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Individual packaging, other sizes and alternative fluorescence intensities (peaks) are available upon request. Please contact our customer service for a custom development.

Selected Publications and References

  • P. Rosendalh, K. Plak, A. Jacobi, M. Kraeter, N. Toepfner, O. Otto, C. Herold, M. Winzi, M. Herbig, Y. Ge, S. Girardo, K. Wagner, B. Baum, J. Guck: Real-time fluorescence and deformability cytometry, 2018, Nature Methods 15, 355-358.
  • "Comparison study of flow cytometers combining novel ultra-bright calibration particles with state-of-the-art characterization methods", K. v. Volkmann, A. Teichmüller, T. Thiele & Toralf Kaiser. (download poster here)