Spectrum Calibration Microbeads
PolyAn’s Spectrum Calibration Microbeads are designed for calibration and performance validation of flow cytometers and other fluorescence imaging systems at all wavelengths. Each bead contains a mixture of highly photostable fluorophores that are homogeneously encapsulated within the PMMA matrix.
The Spectrum Calibration Microbeads can be excited at any wavelength from 365–650 nm and emit at 400–750 nm, which enables their detection in all common fluorescence detection channels with only minimal photobleaching.
Products
Spectrum Calibration Microbeads (8-peak) consisting of one transparent population (only detectable in the PerCP/PE-Cy5 channel, due to the low autofluorescence of PMMA) and 7 fluorescent populations with increasing fluorophore content for all detection channels (measured with a Quantum Analysis Quantum-P flow cytometer).
Key features
- Contains a mixture of fluorophores that enables the Spectrum Calibration Beads to be excited at any wavelength from 365–650 nm.
- Fluorophores are homogeneously encapsulated in the PMMA matrix. Special shell prevents leaching of fluorophores.
- Allows the calibration of the DAPI, FITC, PE, PE-TR, PE-Cy5, and APC channels with the same set of particles.
- Set of up to 8 similar size microparticle populations (peaks) with different fluorescence intensities. The fluorescence intensities in separate channels can be tailored to specific requirements, if needed.
Individual packaging, other sizes and alternative fluorescence intensities (peaks) are available upon request. Please contact our customer service for a custom development.
Selected Publications and References
- Rosendahl, P. et al., `Real-time fluorescence and deformability cytometry´, Nat. Methods, 2018, 15, 355. DOI: 10.1038/nmeth.4639.
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Volkmann, K.v. et al., "Comparison study of flow cytometers combining novel ultra-bright calibration particles with state-of-the-art characterization methods" (download poster here)