Functionalized Coverslips
For high-resolution microscopic imaging or scanning applications PolyAn offers functionalized coverslips. All of PolyAn's standard functional coatings can also be applied to coverslip glass.
All coverslips have the standard dimension of 25 x 60mm. Type #1.5 is used as a standard thickness. All coverslips are packed in boxes of 5. Other functionalities, thicknesses and sizes are available upon request. PolyAn also offers functionalised substrates that are coated with gold or other noble metals as well as waveguide materials.
2D-functionalized Coverslips
For cost-sensitive applications PolyAn has developed a range of 2D (2-dimensional)-reactive coverslips that are manufactured from high quality glass with an ultra-flat surface and low inherent fluorescence. The glass is coated with a thin silane layer that will covalently bind most types of bio-molecules. The defect-free surface features uniform silane layers that provide a high covalent coupling efficiency together with a very low background.
For DNA combing applications PolyAn also offers functionalized coverslips. You can find more information about this specialty product here.
3D-functionalized Coverslips
3D-Surfaces made by Molecular Surface Engineering
PolyAn’s 3D-functionalised microarray Slides are functionalized with a three-dimensional (3D)-surface chemistry comprised of a long-chain polymer containing a defined number of reactive groups. This polymer is covalently linked to the surface of the coverslip.
Our MSE-technology gently binds the functional layer onto the surface without damaging the base substrate. The morphology of the functional surface and thus the number of the reactive groups can be fine-tuned within a narrow range. This yields a number of advantages:
| Low fluorescence background | Covalent binding of functional layer on the substrate without changing the initial autofluorescence. |
| Low unspecific binding | Combination of reactive functional groups with PolyAn antifouling matrix. |
| Topography | Tuneable surface hydrophilicity / hydrophobicity (contact angle). |
| Uniform spot morphology | Narrow variation of surface properties and homogeneous distribution of reactive groups. |
| Optimal density and high accessibility of reactive groups | Morphology and thickness of functional layer tailored to the desired application. |
Technical specifications
Packaging:
The coverslips are packaged in inert atmospheric boxes, with a capacity of 5 coverslips per box.
Storage
PolyAn’s coverslips are characterised by a long shelf-life when stored dry, at 4-8°C and protected from sunlight. All coverslips are packaged in boxes under Argon atmosphere to avoid contamination with particles. The Argon atmosphere also minimises degradation of the reactive surface through contact with air or humidity.