Spectrum Calibration Nanobeads
PolyAn’s Spectrum Calibration Nanobeads can help to adjust fluorescence-based detection systems such as flow cytometers, conventional fluorescence microscopes, confocal laser scanning microscopes, super resolution microscopes, and other image-processing systems.
PolyAn’s Spectrum Calibration Nanobeads are particularly useful to calibrate imaging setups for multi-color applications and to check their capability to resolve and co-localize very small fluorescent objects of different color in the same optical plane.
As Spectrum Calibration Nanobeads resemble the size of extracellular vesicles (EVs), they can also be used to adjust flow cytometers for EV detection. PMMA-Nanobeads compare favorably with polystyrene particles due to their lower refractive index (RI), which is closer to the RI of EVs.
Spectrum Calibration Nanobeads (having a diameter of ca. 575 nm) as measured with a Quantum-P flow cytometer (Quantum Analysis).
Key features
PolyAn's Spectrum Calibration Nanobeads are monodisperse PMMA sub-micron particles that are available in different sizes with diameters between 100 nm and 600 nm. The beads contain a mixture of highly photostable fluorescent dyes that are homogeneously encapsulated within the PMMA matrix.
The Spectrum Calibration Nanobeads can be excited at any wavelength from 365–650 nm and emit at 400–750 nm, which enables their detection in all common fluorescence detection channels (DAPI, FITC, PE/TAMRA/Cy3®, APC/Cy5®, Cy7®) with only minimal photobleaching.
Products
The Spectrum Calibration Nanobeads are also available in other sizes (between 100–600 nm) and with various surface functionalities (e.g. Azide, DBCO, Protein A/G, Neutravidin ...). Alternative fluorescence intensities are available upon request.
Please do not hesitate to contact our customer service for a custom development.