Covalent binding surfaces for Immunoassays
PolyAn´s Amine-binding microplates are useful for immobilizing biomolecules that ineffciently coat by passive adsorption. PolyAn offers amine-binding and nucleophilic binding surfaces, providing a convenient method to covalently immobilize biomolecules, e.g. proteins, peptides, DNA or other ligands.
Amine binding plates
3D-Epoxy 96 well plate, white, 12 x 8-strip, flat bottom
00 680 251
3D-NHS 96 well plate, white, 12 x 8-strip, flat bottom
00 680 451
3D-Epoxy 96 well plate ELISA, C-bottom, transparent, 12 x 8 strip
00 695 251
3D-NHS 96 well plate ELISA, C-bottom, transparent, 12 x 8 strip
00 695 451
PolyAn's 3D-NHS functionalised 96-well microplates are used for covalent immobilisation of biomolecules. The NHS-ester reacts immediately with the NH2- terminus of biochemical species to form a covalent bond with the surface (420 kJ/mol).
PolyAn's 3D-NHS Amine Binding plates are designed to specifically bind primary amines. They are designed to overcome the inherent issues of passive adsorption for immobilizing peptides, proteins, DNA and other ligands for binding assays.
The three-dimensional functional matrix allows the development of covalent binding of nucleophiles to the plate surface without complex coupling chemistry.
- Minimal leaching
- Withstands rigorous washing
- Coating with lower amounts of reagent may be possible
- Control of orientation and even directed (bio-orthogonal) immobilization possible
The structure of the branched, spacered 3-dimensional polymer matrix, which has a thickness of up to 50 nm, also reduces unspecific binding to a minimum.
The plates are offered for colorimetric, chemiluminescence and fluorescent detection systems, respectively. Please go to this section for glass bottom plates with a wide range of surfaces and well designs. Additionally, PolyAn offers functionalized, solvent stable microplates that are comprised of Polypropylene (PP). Please do not hesitate to contact us, if you are looking for solvent stable microplates.
Customised plate surfaces
Please do not hesitate to contact us, if you require a special surface for binding of your biomolecules that is not listed in the products table. We can also functionalise other plate formats and substrates with our surfaces. Additionally, we have access to a wide range of different surface modifications for binding of small molecules, saccharides etc.