Protein Microarrays are two-dimensional arrays of protein capture moieties (e.g. antibodies) printed onto a solid substrate used to assay a large number of analytes (e.g. antigens) within a sample.
Protein microarrays come in two basic formats, "forward phase" (i.e. one antibody probing multiple samples) and "reverse phase" (i.e. one sample probed by multiple capture reagents). In "forward phase" arrays, captured reagents (antibodies, aptamers, or other proteins) are spotted at defined positions on a substrate, which is then interrogated with any of a variety of probes, from protein lysate and enzymes to small molecules and nucleic acids. In "reverse phase" arrays, on the other hand, protein lysates are spotted, and the arrays are then probed with antibodies to detect e.g. phosphorylated signaling molecules.
All of PolyAn’s reactive surfaces are completely transparent. They are characterized by a low lot-to-lot variation that is specified and monitored by using contact angle measurements as well as qualitative test methods.
A flyer showing the most commonly used functionalized surfaces for Protein Microarrays available from PolyAn can be downloaded here.
Surface test package (1) with 3x5 glass slides for immobilization of proteins
|Id||104 00 950|
|Title||Surface test package (1) with 3x5 glass slides for immobilization of proteins|
|Surface Modifications||2D-Epoxy, 2D-Aldehyde and 3D-NHS|
|Product Dimensions||25 mm x 75 mm|
|Packaging Volume||15 Slides/Pack|
|Hts Code||70 17 9 000|
|Product Thickness||1 mm|
|Description||Selection of a wide range of different surface chemistries that are used for immobilization of proteins. The test package is comprised of 5 x 2D-Epoxy glass slides, 5 x 2D-Aldehyde glass slides and 5 x 3D-NHS glass slides. The packages includes standard handling protocols for the different surfaces.|
More hydrophobic surfaces may result in reduced spot diameter, depending on the spotting buffer composition. Results may vary based on buffers, sample preparation, spotting and scanning instruments.
PolyAn is the distributor of the nitrocellulose film slides from Grace Bio-Labs. The following table illustrates the different product families for Reverse-Phase Protein Arrays (RPPA) and other microarray applications:
|Binding Capacity ||++++||++||++++||+|
|Dynamic range (log scale fluorescence)||5-6||5-6||7+||4-5|
|Applications||Best for any application requiring high binding capacity and colorimetric detection.||Reduced fluorescence background with lower binding capacity than AVID. Good signal-to-noise ratio for fluorescence detection.||Second generation NOVA, lowest fluorescence background, high binding capacity. Best for fluorescence detection and large dynamic range.||Lowest fluorescence background, lower binding capacity, reduced dynamic range. Best signal-to-noise ratio for fluorescence detection.|
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