3D-Azide Microplates
for reaction with Alkyne groups or DBCO-modified molecules via Click chemistry
PolyAn’s 3D-Azide Microplates can be applied for bio-orthogonal binding of molecules that contain either Alkyne groups in a Copper-catalyzed Alkyne-Azide Cycloaddition, or Dibenzocyclooctyne (DBCO) groups in a Strain-promoted Alkyne-Azide Cycloaddition.
Products
3D-Azide 12x 8-tube PCR strips (GBO #673210), PP, 100 µL/well functionalization volume
| Id | 008 80 601 |
| Title | 3D-Azide 12x 8-tube PCR strips (GBO #673210), PP, 100 µL/well functionalization volume |
| Substrate | PP strip |
| Format | 12x 8-tube PCR strips, PP |
| Mean Diameter | |
| Color Labeling | |
| Surface Modifications | 3D-Azide |
| Solids Content | |
| Product Dimensions | 85 x 128 x 14 mm |
| Packaging | |
| Packaging Volume | 4 Plates/Box |
| Package Weight | 158 g |
| Dimensions | 128 x 86 x 45 mm |
| Hts Code | 39 26 90 97 |
| Pads Wells | 96 |
| Pad Size | |
| Well Format | PCR 8-tube strips |
| Product Thickness | |
| Description | for Click chemistry reaction with Alkyne- or DBCO-functionalized (bio)molecules |
| Image |
PolyAn also equips glass slides, coverslips, polymer slides, and COP films with reactive 3D-surfaces. Please do not hesitate to contact us, if you would like to functionalize a different format or substrate with our 3D-Azide surface.
3D-Azide surface for DNA hybridization
Measurements to demonstrate and verify the efficient immobilization of DBCO-modified oligonucleotides to 3D-Azide microplates via copper-free click-chemistry.
Left: Immobilization of a 5’-DBCO-oligonucleotide (20mer, 3'-labelled with 6-FAM) via strain promoted alkyne-azide cycloaddition (SPAAC), Right: Hybridization with the corresponding anti-strand (5’-labelled with TAMRA).