for an oriented covalent coupling of thiolated biochemical species
This covalent coupling method is suitable for the oriented or orthogonal coupling of thiol modified probes or biochemical species.
Only thiol groups can be reacted with the double bond of the Maleimide active esters. Similar to a NHS-modfication the esters that are formed are susceptible against hydrolysis. Thiolated probes or biochemical species can contain disulfides bridged dimers and must be reduced e.g. by using Dithiothreitol (DTT) or Cleland´s reagent for an optimal and effective surface coupling.
PolyAn equips glass slides and polymer slides with 3D-Thiol surfaces. Please do not hesitate to contact us, if you would like to functionalize a different format or substrate with our 3D-Thiol surface.