for coupling via the N-terminus of biochemical species
Epoxides are cyclic ethers with a highly strained three member ring. Epoxy rings can be easily reacted with nucleophiles e.g. amines, hydrazines, thiols, hydroxides and carboxyl groups. Compared to NHS-esters or 1,4-Phenylene isothiocyanates (PDITC) the epoxy surface is more stable and has a longer shelf-life. Epoxy-surfaces are stable up temperatures of 40°C and are also more stable against humidity compared to NHS and PDITC-surfaces.
The nucleophilic addition is catalyzed by acid or basic conditions. Under acidic conditions, the oxygen in the ring is positively charged, which facilitates the nucleophilic attack. Under basic conditions the least substituted carbon is attacked by the applied nucleophile in a standard SN2 reaction.
PolyAn equips glass slides and polymer slides as well as 96-well plates with 3D-Epoxy surfaces. The 3D-Epoxy 96 well microplates are used mainly if adsorptive binding of peptides or oligonucleotides, for example, to high/medium binding surfaces is ineffective or the binding strength is not sufficient. Areas of application include detection methods such as ELISA, ELI-Spot, protein and peptide arrays and DNA binding.
Please do not hesitate to contact us, if you would like to functionalize a different format or substrate with our 3D-Epoxy surface.