for coupling via the N-terminus of the biochemical species
Aldehyde groups react immediately with the NH2-terminus of biochemical species to form a covalent bond with the surface (420 kJ/mol). In an intermediate state the Aldehydes form an instable Imine-group with the Amines (Schiff-base). Therefore, we recommend reducing the Imines with Sodium Borhydride to form stable Amines.
Upon completion the coupling reaction other non-reacted aldehydes must be blocked with small molecules that penetrate the 3D-Matrix and effectively quench all remaining reactive groups. If Amines are used for blocking, please again reduce the Imines with Sodium Borhydride to form stable Amines.
PolyAn equips glass slides and polymer slides with 3D-Aldehyde surfaces. Please do not hesitate to contact us, if you would like to functionalize a different format or substrate with our 3D-Aldehyde surface.