PolyAn is offering a portfolio of monodisperse PMMA (poly methyl methacrylate) microparticles for multiplex bead assays, calibration of flow cytometers and calibration of fluorescence imaging systems as well as a wide range of other applications in LifeScience research. PolyAn’s non-porous microparticles are comprised of a PMMA core with a 3D surface modification:

With PolyAn’s production process up to two fluorophores are incorporated into the beads during the polymerisation process. This ensures a much more homogeneous distribution of the dyes within the beads when compared to conventional diffusion controlled dyeing processes. Additionally, the fuorophores are caged within the polymeric PMMA matrix and are less likely to leak-out. The homogenous distribution of fluorophores is illustrated in the adjacent CLSM image.

* Image courtesy of Bundesanstalt für Materialforschung und -prüfung

PolyAn’s carboxy-microparticles (COOH) are functionalised using our proprietary Molecular Surface Engineering (MSE) Technology. PolyAn’s 3D-Carboxy matrix is suitable as a platform for a wide range of coupling methods. Our microparticles are characterised by low non-specific adsorption and low aggregation behaviour.

While we publish an overview of our products, it is by no means comprehensive. If the particle you require is not listed, we urge you to contact us to check our complete inventory. Please note, that we also offer a customization service for your specific application.

Microparticle Characteristics

Our polymer microparticles are based on a PMMA (poly methyl methacrylate) core with a covalently bound reactive 3-dimensional surface.

Using PMMA ensures an excellent optical brilliance and a low autofluorescence compared to other microparticle materials. The refractive index of 1.48 is close to the refractive index of cells (ca. 1.38). Our microparticles have a density of 1.18 g/cm³ and a glass transition temperature (Tg) of about 110°C. PolyAn’s PMMA is biocompatible.

PolyAn produces microparticles in the range between 2-20µm. Each bead population is monodisperse with a maximum Coefficient of Variation (CV) of less than 5%.

Imagesources: *Agricultural Research Service, Wikimedia Commons, **Medipan GmbH, ***Universität Potsdam