Streptavidin and Neutravidin Surfaces

for non-covalent oriented coupling of Biotin modified biochemical species

Streptavidin is a glycoprotein comprised of four polypeptides that are connected with carbohydrates via glycosidic bonds. Streptavidin and Neutravidin are tetrameric proteins which form a highly specific binding site for Biotin. Neutravidin is a deglycosylated form of avidin.

Streptavidin and Neutravidin coated plates

Id Title
00 681 251 Covalently coated Streptavidin 96 well plate, white, 12 x 8-strip, flat bottom
00 682 251 Covalently coated Neutravidin 96 well plate, white, 12 x 8-strip, flat bottom
00 692 251 Covalently coated Streptavidin 96 well transparent, 12 x 8-strip, flat bottom
00 693 251 Covalently coated Neutravidin 96 well transparent, 12 x 8-strip, flat bottom
00 697 451 Covalently coated Streptavidin 96 well plate ELISA, C-bottom, transparent, 12 x 8 strip, 100 µL functionalisation volume Info
00 698 451 Covalently coated Neutravidin 96 well plate ELISA, C-bottom, transparent, 12 x 8 strip, 100 µL functionalisation volume Info
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The Avidin (Streptavidin/Neutravidin) -Biotin-bond is one of the strongest known, non-covalent bond in biology/ biochemistry (KD= 10-15 mol/l*). The binding site for Biotin is formed by various amino acids (see adjacent figure: binding site). When using covalently attached Avidin, Streptavidin or Neutravidin the molecules are less susceptible for desorption in the presence of alkaline, acids, in solutions of high ionic strength or at high temperatures. Biotin affine proteins can be distinguished by their isoelectric point, specificity and nonspecific binding as illustrated in the following table.

AvidinStreptavidinNeutravidin
Molecular Weight67 kDa53 kDa60 kDa
Biotin Binding Sites444
Isoelectric Point (pl)106.8-7.56.3
SpecificityLowHighHighest
Affinity for Biotin (Kd)10-15 M10-15 M10-15 M
Nonspecific BindingHighLowLowest

PolyAn also equips glass slides and polymer slides with Streptavidin and Neutravidin surfaces.

Please do not hesitate to contact us, if you would like to functionalize a different format or substrate with our Streptavidin or Neutravidin surfaces.