Amine binding plates for ELISA
PolyAn´s Amine-binding microplates are useful for immobilizing biomolecules that ineffciently coat by passive adsorption. PolyAn offers amine-binding and nucleophilic binding surfaces, providing a convenient method to covalently immobilize biomolecules, e.g. proteins, peptides, amino-modifed oligonucleotides or other ligands.
3D-Epoxy and 3D-NHS functionalized ELISA microplates
3D-Epoxy 96 well plate, white, 12 x 8-strip, flat bottom, 100 µL functionaization volume
00 680 251
3D-NHS 96 well plate, white, 12 x 8-strip, flat bottom
00 680 451
3D-Epoxy 96 well plate ELISA, C-bottom, transparent, 12 x 8 strip, 100 µL functionalisation volume
00 695 251
3D-NHS 96 well plate ELISA, C-bottom, transparent, 12 x 8 strip, 100 µL functionalisation volume
00 695 451
PolyAn's 3D-NHS functionalised 96-well microplates are used for covalent immobilisation of biomolecules. The NHS-ester reacts immediately with the NH2- terminus of biochemical species to form a covalent bond with the surface (420 kJ/mol).
PolyAn's 3D-NHS Amine Binding plates are designed to specifically bind primary amines of peptides, proteins and other molecules. They are designed to overcome the inherent issues of passive adsorption for immobilizing peptides and other ligands for binding assays.
The three-dimensional functional matrix allows the development of covalent binding of nucleophiles to the plate surface without complex coupling chemistry.
- branched, spacered 3-dimensional polymer
- simple coupling chemistry
- directed (bio-orthogonal) immobilization possible
- economic alternative to streptavidin coated plates
The structure of the matrix, which has a thickness of up to 50 nm, also reduces unspecific binding to a minimum.
The plates are offered for colorimetric, chemiluminescence and fluorescent detection systems, respectively. PolyAn can also offer functionalized, solvent stable microplates that are comprised of Polypropylene (PP). Please do not hesitate to contact us, if you are looking for solvent stable microplates.
ELISA application: IgG-FITC immobilization on PolyAn 3D-surfaces versus a passive/adsorptive binding surface
Customised plate surfaces
Please do not hesitate to contact us, if you require a special surface for binding of your biomolecules that is not listed in the products table. We can also functionalise other plate formats and substrates with our surfaces. Additionally, we have access to a wide range of different surface modifications for binding of small molecules, saccharides etc.